@article{5899c5f3716c4c41a6e2c68bfe2d1266,
title = "[40] Reduced Nicotinamide Adenine Dinucleotide Phosphate: Δ4-3-Ketosteroid 5α-Oxidoreductase (Rat Ventral Prostate)",
abstract = "The 5α-reduction of radioactive Δ4-3-ketosteroids, which do not contain substitutions on carbon-11 of the steroid molecule, is followed in the presence of an excess of NADPH by measuring the appearance of the radioactive 5α-reduced metabolites, utilizing a rapid thin-layer chromatographic separation. The assay in this chapter has been developed because the low activity of the enzyme in most tissues precludes the measurement of NADPH disappearance spectrophotometrically. The enzyme exhibits distinctive tissue localization in that most activity is found in the organs of accessory reproduction and in liver.",
author = "Moore, {Ronald J.} and Wilson, {Jean D.}",
note = "Funding Information: Buffer, potassium phosphate, 0.1 M, pH 6.6 Stock substrate solution, \[1 ,2-3H\]testosterone, available from several radiochemical sources at activities of approximately 50 Ci/mmole and about 95% purity, is diluted with benzene to a final activity of 0.1 mCi/ml and a concentration of approximately 2 t~M. Tween 40 (1:50 w/v in ethanol) is added to provide 0.5 mg Tween/~g radiolabeled testosterone. The solution, stored at 4 °, is stable for several months * Supported by Grant AM 03892 from the National Institutes of Health. 1 The trivial names used are testosterone, 17fl-hydroxyandrost-4-en-3-one; dihydro-testosterone, 17fl-hydroxy-Sa-androstan-3-one; androstandiol, 5a-androstan-3%lTfl-diol and 5a-androstan-3fl,17fl-diol; epitestosterone, 17a-hydroxyandrost-4-en-3-one ; 17a-hydroxyprogesterone, 17a-hydroxypregn-4-ene-3,20-dione; progesterone, pregn-4-ene-3,20-dione ; deoxycorticosterone, 21-hydroxypregn-4-ene-3,20-dione; cortexo-lone, 17~,21-dihydroxy-pregn-4-ene, 3,20-dione; androstenedione, androst-4-ene,3-17-dione; androstenediol, androst-4-ene,3fl,17~-diol; corticosterone, llfl,21-dihydroxy-pregn-4-ene-3, 20-dione; cortisol, llfl,17a,21-trihydroxypregn-4-ene-3,20-dione; corti-sone, 17a,21-dihydroxypregn-4-ene,3,11,20-trione.",
year = "1975",
month = jan,
day = "1",
doi = "10.1016/S0076-6879(75)36043-6",
language = "English (US)",
volume = "36",
pages = "466--474",
journal = "Methods in Enzymology",
issn = "0076-6879",
publisher = "Academic Press Inc.",
number = "C",
}