5-Azacytidine induced myogenesis in a differentiation defective cell line

C. Walker, J. W. Shay

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

A differentiation defective cell line variant, the T984-15, has lost the capacity to differentiate myogenically. Following treatment with the hypomethylating agent 5-azacytidine, T984-15 cells were induced to differentiate into myogenic colonies containing fused myotubes. Myogenic colonies when cloned, maintained their ability to differentiate after prolonged culture in the absence of further 5-azacytidine treatment. These results indicate that 5-azacytidine treatment resulted in a stable alteration in the capacity of T984-15 cells to differentiate and suggests that the loss of myogenic potential may have occurred as a result of an epigenetic phenomenon rather than a somatic mutational event.

Original languageEnglish (US)
Pages (from-to)259-263
Number of pages5
JournalDifferentiation
Volume25
Issue number1-3
DOIs
StatePublished - 1984

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology
  • Cell Biology
  • Cancer Research

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