5mC oxidation by Tet2 modulates enhancer activity and timing of transcriptome reprogramming during differentiation

Gary C. Hon, Chun Xiao Song, Tingting Du, Fulai Jin, Siddarth Selvaraj, Ah Young Lee, Chia An Yen, Zhen Ye, Shi Qing Mao, Bang An Wang, Samantha Kuan, Lee E. Edsall, Boxuan Simen Zhao, Guo Liang Xu, Chuan He, Bing Ren

Research output: Contribution to journalArticle

141 Citations (Scopus)

Abstract

In mammals, cytosine methylation (5mC) is widely distributed throughout the genome but is notably depleted from active promoters and enhancers. While the role of DNA methylation in promoter silencing has been well documented, the function of this epigenetic mark at enhancers remains unclear. Recent experiments have demonstrated that enhancers are enriched for 5-hydroxymethylcytosine (5hmC), an oxidization product of the Tet family of 5mC dioxygenases and an intermediate of DNA demethylation. These results support the involvement of Tet proteins in the regulation of dynamic DNA methylation at enhancers. By mapping DNA methylation and hydroxymethylation at base resolution, we find that deletion of Tet2 causes extensive loss of 5hmC at enhancers, accompanied by enhancer hypermethylation, reduction of enhancer activity, and delayed gene induction in the early steps of differentiation. Our results reveal that DNA demethylation modulates enhancer activity, and its disruption influences the timing of transcriptome reprogramming during cellular differentiation.

Original languageEnglish (US)
Pages (from-to)286-297
Number of pages12
JournalMolecular Cell
Volume56
Issue number2
DOIs
StatePublished - Jan 1 2014

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DNA Methylation
Transcriptome
Dioxygenases
Cytosine
DNA
Epigenomics
Methylation
Mammals
Genome
Genes
Proteins
5-hydroxymethylcytosine

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

5mC oxidation by Tet2 modulates enhancer activity and timing of transcriptome reprogramming during differentiation. / Hon, Gary C.; Song, Chun Xiao; Du, Tingting; Jin, Fulai; Selvaraj, Siddarth; Lee, Ah Young; Yen, Chia An; Ye, Zhen; Mao, Shi Qing; Wang, Bang An; Kuan, Samantha; Edsall, Lee E.; Zhao, Boxuan Simen; Xu, Guo Liang; He, Chuan; Ren, Bing.

In: Molecular Cell, Vol. 56, No. 2, 01.01.2014, p. 286-297.

Research output: Contribution to journalArticle

Hon, GC, Song, CX, Du, T, Jin, F, Selvaraj, S, Lee, AY, Yen, CA, Ye, Z, Mao, SQ, Wang, BA, Kuan, S, Edsall, LE, Zhao, BS, Xu, GL, He, C & Ren, B 2014, '5mC oxidation by Tet2 modulates enhancer activity and timing of transcriptome reprogramming during differentiation', Molecular Cell, vol. 56, no. 2, pp. 286-297. https://doi.org/10.1016/j.molcel.2014.08.026
Hon, Gary C. ; Song, Chun Xiao ; Du, Tingting ; Jin, Fulai ; Selvaraj, Siddarth ; Lee, Ah Young ; Yen, Chia An ; Ye, Zhen ; Mao, Shi Qing ; Wang, Bang An ; Kuan, Samantha ; Edsall, Lee E. ; Zhao, Boxuan Simen ; Xu, Guo Liang ; He, Chuan ; Ren, Bing. / 5mC oxidation by Tet2 modulates enhancer activity and timing of transcriptome reprogramming during differentiation. In: Molecular Cell. 2014 ; Vol. 56, No. 2. pp. 286-297.
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