A cellulose synthase-like protein is required for osmotic stress tolerance in Arabidopsis

Jianhua Zhu, Byeong Ha Lee, Mike Dellinger, Xinping Cui, Changqing Zhang, Shang Wu, Eugene A. Nothnagel, Jian Kang Zhu

Research output: Contribution to journalArticle

64 Citations (Scopus)

Abstract

Osmotic stress imposed by soil salinity and drought stress significantly affects plant growth and development, but osmotic stress sensing and tolerance mechanisms are not well understood. Forward genetic screens using a root-bending assay have previously identified salt overly sensitive (sos) mutants of Arabidopsis that fall into five loci, SOS1 to SOS5. These loci are required for the regulation of ion homeostasis or cell expansion under salt stress, but do not play a major role in plant tolerance to the osmotic stress component of soil salinity or drought. Here we report an additional sos mutant, sos6-1, which defines a locus essential for osmotic stress tolerance. sos6-1 plants are hypersensitive to salt stress and osmotic stress imposed by mannitol or polyethylene glycol in culture media or by water deficit in the soil. SOS6 encodes a cellulose synthase-like protein, AtCSLD5. Only modest differences in cell wall chemical composition could be detected, but we found that sos6-1 mutant plants accumulate high levels of reactive oxygen species (ROS) under osmotic stress and are hypersensitive to the oxidative stress reagent methyl viologen. The results suggest that SOS6/AtCSLD5 is not required for normal plant growth and development but has a critical role in osmotic stress tolerance and this function likely involves its regulation of ROS under stress.

Original languageEnglish (US)
Pages (from-to)128-140
Number of pages13
JournalPlant Journal
Volume63
Issue number1
DOIs
StatePublished - Jul 2010

Fingerprint

cellulose synthase
Osmotic Pressure
osmotic stress
Arabidopsis
stress tolerance
Salts
Proteins
proteins
salt stress
Plant Development
Soil
Droughts
Salinity
soil salinity
Growth and Development
mutants
loci
plant development
reactive oxygen species
Reactive Oxygen Species

Keywords

  • Arabidopsis
  • cell wall
  • cellulose synthase-like protein
  • osmotic stress tolerance
  • reactive oxygen species
  • SOS6

ASJC Scopus subject areas

  • Plant Science
  • Cell Biology
  • Genetics
  • Medicine(all)

Cite this

A cellulose synthase-like protein is required for osmotic stress tolerance in Arabidopsis. / Zhu, Jianhua; Lee, Byeong Ha; Dellinger, Mike; Cui, Xinping; Zhang, Changqing; Wu, Shang; Nothnagel, Eugene A.; Zhu, Jian Kang.

In: Plant Journal, Vol. 63, No. 1, 07.2010, p. 128-140.

Research output: Contribution to journalArticle

Zhu, Jianhua ; Lee, Byeong Ha ; Dellinger, Mike ; Cui, Xinping ; Zhang, Changqing ; Wu, Shang ; Nothnagel, Eugene A. ; Zhu, Jian Kang. / A cellulose synthase-like protein is required for osmotic stress tolerance in Arabidopsis. In: Plant Journal. 2010 ; Vol. 63, No. 1. pp. 128-140.
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