A common motif targets huntingtin and the androgen receptor to the proteasome

Shweta Chandra, Jieya Shao, Jennifer X. Li, Mei Li, Frank M. Longo, Marc I. Diamond

Research output: Contribution to journalArticle

15 Scopus citations

Abstract

Huntington disease derives from a critically expanded polyglutamine tract in the huntingtin (Htt) protein; a similar polyglutamine expansion in the androgen receptor (AR) causes spinobulbar muscular atrophy. AR activity also plays an essential role in prostate cancer. Molecular mechanisms that regulate Htt and AR degradation are not well understood but could have important therapeutic implications. We find that a pentapeptide motif (FQKLL) within the Htt protein regulates its degradation and subcellular localization to cytoplasm puncta. Disruption of the motif by alanine substitution at the hydrophobic residues increases the steady state level of the protein. Pulse-chase analyses indicate that the motif regulates degradation. A similar motif (FQNLF) has corresponding activities in the AR protein. Transfer of the Htt motif with five flanking amino acids on either side to YFP reduces the steady state YFP level by rendering it susceptible to proteasome degradation. This work defines a novel proteasome-targeting motif that is necessary and sufficient to regulate the degradation of two disease-associated proteins.

Original languageEnglish (US)
Pages (from-to)23950-23955
Number of pages6
JournalJournal of Biological Chemistry
Volume283
Issue number35
DOIs
Publication statusPublished - Aug 29 2008

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ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

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