A Cytosine Methytransferase Modulates the Cell Envelope Stress Response in the Cholera Pathogen

Michael C. Chao, Shijia Zhu, Satoshi Kimura, Brigid M. Davis, Eric E. Schadt, Gang Fang, Matthew K. Waldor

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

DNA methylation is a key epigenetic regulator in all domains of life, yet the effects of most bacterial DNA methyltransferases on cellular processes are largely undefined. Here, we used diverse techniques, including bisulfite sequencing, transcriptomics, and transposon insertion site sequencing to extensively characterize a 5-methylcytosine (5mC) methyltransferase, VchM, in the cholera pathogen, Vibrio cholerae. We have comprehensively defined VchM’s DNA targets, its genetic interactions and the gene networks that it regulates. Although VchM is a relatively new component of the V. cholerae genome, it is required for optimal V. cholerae growth in vitro and during infection. Unexpectedly, the usually essential σ E cell envelope stress pathway is dispensable in ∆vchM V. cholerae, likely due to its lower activation in this mutant and the capacity for VchM methylation to limit expression of some cell envelope modifying genes. Our work illuminates how an acquired DNA methyltransferase can become integrated within complex cell circuits to control critical housekeeping processes.

Original languageEnglish (US)
Article numbere1005666
JournalPLoS genetics
Volume11
Issue number11
DOIs
StatePublished - Nov 2015
Externally publishedYes

Fingerprint

cholera
Vibrio cholerae
Cholera
cytosine
Cytosine
stress response
methyltransferases
Methyltransferases
pathogen
pathogens
DNA
methylation
cells
5-Methylcytosine
bisulfites
Housekeeping
Bacterial DNA
Gene Regulatory Networks
DNA methylation
DNA Methylation

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Molecular Biology
  • Genetics
  • Genetics(clinical)
  • Cancer Research

Cite this

Chao, M. C., Zhu, S., Kimura, S., Davis, B. M., Schadt, E. E., Fang, G., & Waldor, M. K. (2015). A Cytosine Methytransferase Modulates the Cell Envelope Stress Response in the Cholera Pathogen. PLoS genetics, 11(11), [e1005666]. https://doi.org/10.1371/journal.pgen.1005666

A Cytosine Methytransferase Modulates the Cell Envelope Stress Response in the Cholera Pathogen. / Chao, Michael C.; Zhu, Shijia; Kimura, Satoshi; Davis, Brigid M.; Schadt, Eric E.; Fang, Gang; Waldor, Matthew K.

In: PLoS genetics, Vol. 11, No. 11, e1005666, 11.2015.

Research output: Contribution to journalArticle

Chao, Michael C. ; Zhu, Shijia ; Kimura, Satoshi ; Davis, Brigid M. ; Schadt, Eric E. ; Fang, Gang ; Waldor, Matthew K. / A Cytosine Methytransferase Modulates the Cell Envelope Stress Response in the Cholera Pathogen. In: PLoS genetics. 2015 ; Vol. 11, No. 11.
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