A demonstration of several deoxyribonuclease activities in mammalian cell mitochondria

M. Durphy; P. N. Manley; E. C. Friedberg

doi:10.1083/jcb.62.3.695

A demonstration of several deoxyribonuclease activities in mammalian cell mitochondria

M. Durphy, P. N. Manley, E. C. Friedberg

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18 Scopus citations

Abstract

Extracts of purified mitochondria from adult rabbit liver and kidney have been prepared by lysis with Triton X-100. Such extracts contain deoxyribonuclease activity demonstrable at alkaline pH. Studies utilizing the effects of substrate variation, differing ionic strength, nucleoside di-and triphosphates, and SH-group inhibitors reveal the existence of at least five distinguishable deoxyribonuclease activities in these extracts. Assay of lysosomal and mitochondrial enzyme markers indicates no significant lysosomal contamination of the mitochondrial extracts. Further studies also suggest that the alkaline deoxyribonuclease activity is specifically located in or in association with mitochondria.

Original language	English (US)
Pages (from-to)	695-706
Number of pages	12
Journal	Journal of Cell Biology
Volume	62
Issue number	3
DOIs	https://doi.org/10.1083/jcb.62.3.695
State	Published - Sep 1 1974

ASJC Scopus subject areas

Cell Biology

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10.1083/jcb.62.3.695

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A demonstration of several deoxyribonuclease activities in mammalian cell mitochondria

Abstract

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