A lathe system for micrometre-sized cylindrical sample preparation at room and cryogenic temperatures

Mirko Holler; Johannes Ihli; Esther H.R. Tsai; Fabio Nudelman; Mariana Verezhak; Wilma D.J. Van De Berg; Sarah H. Shahmoradian

doi:10.1107/S1600577519017028

A lathe system for micrometre-sized cylindrical sample preparation at room and cryogenic temperatures

Mirko Holler, Johannes Ihli, Esther H.R. Tsai, Fabio Nudelman, Mariana Verezhak, Wilma D.J. Van De Berg, Sarah H. Shahmoradian

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

A simple two-spindle based lathe system for the preparation of cylindrical samples intended for X-ray tomography is presented. The setup can operate at room temperature as well as under cryogenic conditions, allowing the preparation of samples down to 20 and 50μm in diameter, respectively, within minutes. Case studies are presented involving the preparation of a brittle biomineral brachiopod shell and cryogenically fixed soft brain tissue, and their examination by means of ptychographic X-ray computed tomography reveals the preparation method to be mainly free from causing artefacts. Since this lathe system easily yields near-cylindrical samples ideal for tomography, a usage for a wide variety of otherwise challenging specimens is anticipated, in addition to potential use as a time-and cost-saving tool prior to focused ion-beam milling. Fast sample preparation becomes especially important in relation to shorter measurement times expected in next-generation synchrotron sources.

Original language	English (US)
Pages (from-to)	472-476
Number of pages	5
Journal	Journal of Synchrotron Radiation
Volume	27
DOIs	https://doi.org/10.1107/S1600577519017028
State	Published - Mar 1 2020
Externally published	Yes

Keywords

Sample preparation
nano-Tomography
ptychography

ASJC Scopus subject areas

Radiation
Nuclear and High Energy Physics
Instrumentation

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10.1107/S1600577519017028

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title = "A lathe system for micrometre-sized cylindrical sample preparation at room and cryogenic temperatures",

abstract = "A simple two-spindle based lathe system for the preparation of cylindrical samples intended for X-ray tomography is presented. The setup can operate at room temperature as well as under cryogenic conditions, allowing the preparation of samples down to 20 and 50μm in diameter, respectively, within minutes. Case studies are presented involving the preparation of a brittle biomineral brachiopod shell and cryogenically fixed soft brain tissue, and their examination by means of ptychographic X-ray computed tomography reveals the preparation method to be mainly free from causing artefacts. Since this lathe system easily yields near-cylindrical samples ideal for tomography, a usage for a wide variety of otherwise challenging specimens is anticipated, in addition to potential use as a time-and cost-saving tool prior to focused ion-beam milling. Fast sample preparation becomes especially important in relation to shorter measurement times expected in next-generation synchrotron sources.",

keywords = "Sample preparation, nano-Tomography, ptychography",

author = "Mirko Holler and Johannes Ihli and Tsai, {Esther H.R.} and Fabio Nudelman and Mariana Verezhak and {Van De Berg}, {Wilma D.J.} and Shahmoradian, {Sarah H.}",

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doi = "10.1107/S1600577519017028",

language = "English (US)",

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T1 - A lathe system for micrometre-sized cylindrical sample preparation at room and cryogenic temperatures

AU - Holler, Mirko

AU - Ihli, Johannes

AU - Tsai, Esther H.R.

AU - Nudelman, Fabio

AU - Verezhak, Mariana

AU - Van De Berg, Wilma D.J.

AU - Shahmoradian, Sarah H.

PY - 2020/3/1

Y1 - 2020/3/1

N2 - A simple two-spindle based lathe system for the preparation of cylindrical samples intended for X-ray tomography is presented. The setup can operate at room temperature as well as under cryogenic conditions, allowing the preparation of samples down to 20 and 50μm in diameter, respectively, within minutes. Case studies are presented involving the preparation of a brittle biomineral brachiopod shell and cryogenically fixed soft brain tissue, and their examination by means of ptychographic X-ray computed tomography reveals the preparation method to be mainly free from causing artefacts. Since this lathe system easily yields near-cylindrical samples ideal for tomography, a usage for a wide variety of otherwise challenging specimens is anticipated, in addition to potential use as a time-and cost-saving tool prior to focused ion-beam milling. Fast sample preparation becomes especially important in relation to shorter measurement times expected in next-generation synchrotron sources.

AB - A simple two-spindle based lathe system for the preparation of cylindrical samples intended for X-ray tomography is presented. The setup can operate at room temperature as well as under cryogenic conditions, allowing the preparation of samples down to 20 and 50μm in diameter, respectively, within minutes. Case studies are presented involving the preparation of a brittle biomineral brachiopod shell and cryogenically fixed soft brain tissue, and their examination by means of ptychographic X-ray computed tomography reveals the preparation method to be mainly free from causing artefacts. Since this lathe system easily yields near-cylindrical samples ideal for tomography, a usage for a wide variety of otherwise challenging specimens is anticipated, in addition to potential use as a time-and cost-saving tool prior to focused ion-beam milling. Fast sample preparation becomes especially important in relation to shorter measurement times expected in next-generation synchrotron sources.

KW - Sample preparation

KW - nano-Tomography

KW - ptychography

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JO - Journal of Synchrotron Radiation

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ER -

A lathe system for micrometre-sized cylindrical sample preparation at room and cryogenic temperatures

Abstract

Keywords

ASJC Scopus subject areas

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