A lophotrochozoan-specific nuclear hormone receptor is required for reproductive system development in the planarian

Marla E. Tharp, James J. Collins, Phillip A. Newmark

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Germ cells of sexually reproducing organisms receive an array of cues from somatic tissues that instruct developmental processes. Although the nature of these signals differs amongst organisms, the importance of germline-soma interactions is a common theme. Recently, peptide hormones from the nervous system have been shown to regulate germ cell development in the planarian Schmidtea mediterranea; thus, we sought to investigate a second class of hormones with a conserved role in reproduction, the lipophilic hormones. In order to study these signals, we identified a set of putative lipophilic hormone receptors, known as nuclear hormone receptors, and analyzed their functions in reproductive development. We found one gene, nhr-1, belonging to a small class of functionally uncharacterized lophotrochozoan-specific receptors, to be essential for the development of differentiated germ cells. Upon nhr-1 knockdown, germ cells in the testes and ovaries fail to mature, and remain as undifferentiated germline stem cells. Further analysis revealed that nhr-1 mRNA is expressed in the accessory reproductive organs and is required for their development, suggesting that this transcription factor functions cell non-autonomously in regulating germ cell development. Our studies identify a role for nuclear hormone receptors in planarian reproductive maturation and reinforce the significance of germline-soma interactions in sexual reproduction across metazoans.

Original languageEnglish (US)
Pages (from-to)150-157
Number of pages8
JournalDevelopmental Biology
Volume396
Issue number1
DOIs
StatePublished - Dec 1 2014

Fingerprint

Planarians
Cytoplasmic and Nuclear Receptors
Germ Cells
Carisoprodol
Hormones
Reproduction
Peptide Hormones
Nervous System
Cues
Testis
Ovary
Transcription Factors
Stem Cells
Messenger RNA
Genes

Keywords

  • Accessory reproductive organ
  • Germ cell
  • Lophotrochozoa
  • Nuclear hormone receptor
  • Planaria

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology
  • Molecular Biology

Cite this

A lophotrochozoan-specific nuclear hormone receptor is required for reproductive system development in the planarian. / Tharp, Marla E.; Collins, James J.; Newmark, Phillip A.

In: Developmental Biology, Vol. 396, No. 1, 01.12.2014, p. 150-157.

Research output: Contribution to journalArticle

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