A MAM7 peptide-based inhibitor of staphylococcus aureus adhesion does not interfere with in vitro host cell function

Catherine Alice Hawley, Charlie Anne Watson, Kim Orth, Anne Marie Krachler

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Adhesion inhibitors that block the attachment of pathogens to host tissues may be used synergistically with or as an alternative to antibiotics. The wide-spread bacterial adhesin Multivalent Adhesion Molecule (MAM) 7 has recently emerged as a candidate molecule for a broad-spectrum adhesion inhibitor which may be used to prevent bacterial colonization of wounds. Here we have tested if the antibacterial properties of a MAM-based inhibitor could be used to competitively inhibit adhesion of methicillin-resistant Staphylococcus aureus (MRSA) to host cells. Additionally, we analyzed its effect on host cellular functions linked to the host receptor fibronectin, such as migration, adhesion and matrix formation in vitro, to evaluate potential side effects prior to advancing our studies to in vivo infection models. As controls, we used inhibitors based on well-characterized bacterial adhesin-derived peptides from F1 and FnBPA, which are known to affect host cellular functions. Inhibitors based on F1 or FnBPA blocked MRSA attachment but at the same time abrogated important cellular functions. A MAM7-based inhibitor did not interfere with host cell function while showing good efficacy against MRSA adhesion in a tissue culture model. These observations provide a possible candidate for a bacterial adhesion inhibitor that does not cause adverse effects on host cells while preventing bacterial infection. Copyright:

Original languageEnglish (US)
JournalPLoS One
Volume8
Issue number11
DOIs
StatePublished - Nov 12 2013

Fingerprint

Methicillin-Resistant Staphylococcus aureus
Bacterial Adhesins
adhesion
Staphylococcus aureus
Adhesion
peptides
Peptides
Integrin alpha5beta1
Bacterial Adhesion
Methicillin
cells
adhesins
Bacterial Infections
Anti-Bacterial Agents
Molecules
adverse effects
bacterial adhesion
Wounds and Injuries
bacterial colonization
Infection

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

A MAM7 peptide-based inhibitor of staphylococcus aureus adhesion does not interfere with in vitro host cell function. / Hawley, Catherine Alice; Watson, Charlie Anne; Orth, Kim; Krachler, Anne Marie.

In: PLoS One, Vol. 8, No. 11, 12.11.2013.

Research output: Contribution to journalArticle

Hawley, Catherine Alice ; Watson, Charlie Anne ; Orth, Kim ; Krachler, Anne Marie. / A MAM7 peptide-based inhibitor of staphylococcus aureus adhesion does not interfere with in vitro host cell function. In: PLoS One. 2013 ; Vol. 8, No. 11.
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