A marker assisted selection protocol (MASP) to generate C57BL/6J or 129S6/SvEvTac speed congenic or consomic strains

Sandi Jo Estill, Joseph A. Garcia

Research output: Contribution to journalArticlepeer-review

16 Scopus citations

Abstract

A marker assisted selection protocol is presented that allows for the generation of congenic or consomic strains derived from a C578L/6J:129S6/ SvEvTac mixed strain background. The protocol uses defined primer pairs to generate amplicons that can be distinguished by non-denaturing agarose electrophoresis. Use of this application should result in substantial savings in time, effort, and cost for investigators in all areas of transgenic mouse research.

Original languageEnglish (US)
Pages (from-to)164-166
Number of pages3
JournalGenesis
Volume28
Issue number3-4
DOIs
StatePublished - Jan 1 2000

Keywords

  • 129S6/SvEvTac
  • C57BL/6J
  • Consomic
  • MASP
  • SSLP
  • Speed congenic

ASJC Scopus subject areas

  • Genetics
  • Endocrinology
  • Cell Biology

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