A marker assisted selection protocol (MASP) to generate C57BL/6J or 129S6/SvEvTac speed congenic or consomic strains

Sandi Jo Estill; Joseph A. Garcia

doi:10.1002/1526-968X(200011/12)28:3/4<164::AID-GENE110>3.0.CO;2-R

A marker assisted selection protocol (MASP) to generate C57BL/6J or 129S6/SvEvTac speed congenic or consomic strains

Sandi Jo Estill, Joseph A. Garcia

Research output: Contribution to journal › Article › peer-review

17 Scopus citations

Abstract

A marker assisted selection protocol is presented that allows for the generation of congenic or consomic strains derived from a C578L/6J:129S6/ SvEvTac mixed strain background. The protocol uses defined primer pairs to generate amplicons that can be distinguished by non-denaturing agarose electrophoresis. Use of this application should result in substantial savings in time, effort, and cost for investigators in all areas of transgenic mouse research.

Original language	English (US)
Pages (from-to)	164-166
Number of pages	3
Journal	Genesis
Volume	28
Issue number	3-4
DOIs	https://doi.org/10.1002/1526-968X(200011/12)28:3/4<164::AID-GENE110>3.0.CO;2-R
State	Published - Jan 1 2000

Keywords

129S6/SvEvTac
C57BL/6J
Consomic
MASP
SSLP
Speed congenic

ASJC Scopus subject areas

Genetics
Endocrinology
Cell Biology

Access to Document

10.1002/1526-968X(200011/12)28:3/4<164::AID-GENE110>3.0.CO;2-R

Cite this

@article{be7f322eb5054b02a6cda6a069e4c353,

title = "A marker assisted selection protocol (MASP) to generate C57BL/6J or 129S6/SvEvTac speed congenic or consomic strains",

abstract = "A marker assisted selection protocol is presented that allows for the generation of congenic or consomic strains derived from a C578L/6J:129S6/ SvEvTac mixed strain background. The protocol uses defined primer pairs to generate amplicons that can be distinguished by non-denaturing agarose electrophoresis. Use of this application should result in substantial savings in time, effort, and cost for investigators in all areas of transgenic mouse research.",

keywords = "129S6/SvEvTac, C57BL/6J, Consomic, MASP, SSLP, Speed congenic",

author = "Estill, {Sandi Jo} and Garcia, {Joseph A.}",

year = "2000",

month = jan,

day = "1",

doi = "10.1002/1526-968X(200011/12)28:3/4<164::AID-GENE110>3.0.CO;2-R",

language = "English (US)",

volume = "28",

pages = "164--166",

journal = "Genesis",

issn = "1526-954X",

publisher = "Wiley-Liss Inc.",

number = "3-4",

}

TY - JOUR

T1 - A marker assisted selection protocol (MASP) to generate C57BL/6J or 129S6/SvEvTac speed congenic or consomic strains

AU - Estill, Sandi Jo

AU - Garcia, Joseph A.

PY - 2000/1/1

Y1 - 2000/1/1

N2 - A marker assisted selection protocol is presented that allows for the generation of congenic or consomic strains derived from a C578L/6J:129S6/ SvEvTac mixed strain background. The protocol uses defined primer pairs to generate amplicons that can be distinguished by non-denaturing agarose electrophoresis. Use of this application should result in substantial savings in time, effort, and cost for investigators in all areas of transgenic mouse research.

AB - A marker assisted selection protocol is presented that allows for the generation of congenic or consomic strains derived from a C578L/6J:129S6/ SvEvTac mixed strain background. The protocol uses defined primer pairs to generate amplicons that can be distinguished by non-denaturing agarose electrophoresis. Use of this application should result in substantial savings in time, effort, and cost for investigators in all areas of transgenic mouse research.

KW - 129S6/SvEvTac

KW - C57BL/6J

KW - Consomic

KW - MASP

KW - SSLP

KW - Speed congenic

UR - http://www.scopus.com/inward/record.url?scp=0034485684&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034485684&partnerID=8YFLogxK

U2 - 10.1002/1526-968X(200011/12)28:3/4<164::AID-GENE110>3.0.CO;2-R

DO - 10.1002/1526-968X(200011/12)28:3/4<164::AID-GENE110>3.0.CO;2-R

M3 - Article

C2 - 11105059

AN - SCOPUS:0034485684

SN - 1526-954X

VL - 28

SP - 164

EP - 166

JO - Genesis

JF - Genesis

IS - 3-4

ER -

A marker assisted selection protocol (MASP) to generate C57BL/6J or 129S6/SvEvTac speed congenic or consomic strains

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this