A marker assisted selection protocol is presented that allows for the generation of congenic or consomic strains derived from a C578L/6J:129S6/ SvEvTac mixed strain background. The protocol uses defined primer pairs to generate amplicons that can be distinguished by non-denaturing agarose electrophoresis. Use of this application should result in substantial savings in time, effort, and cost for investigators in all areas of transgenic mouse research.
|Original language||English (US)|
|Number of pages||3|
|State||Published - Jan 1 2000|
- Speed congenic
ASJC Scopus subject areas
- Cell Biology