A Mef2 gene that generates a muscle-specific isoform via alternative mRNA splicing

James F. Martin, Joseph M. Miano, Carolyn M. Hustad, Neal G. Copeland, Nancy A. Jenkins, Eric N. Olson

Research output: Contribution to journalArticle

177 Citations (Scopus)

Abstract

Members of the myocyte-specific enhancer-binding factor 2 (MEF2) family of transcription factors bind a conserved A/T-rich sequence in the control regions of numerous muscle-specific genes. Mammalian MEF2 proteins have been shown previously to be encoded by three genes, Mef2, xMef2, and Mef2c, each of which gives rise to multiple alternatively spliced transcripts. We describe the cloning of a new member of the MEF2 family from mice, termed MEF2D, which shares extensive homology with other MEF2 proteins but is the product of a separate gene. MEF2D binds to and activates transcription through the MEF2 site and forms heterodimers with other members of the MEF2 family. Deletion mutations show that the carboxyl terminus of MEF2D is required for efficient transactivation. MEF2D transcripts are widely expressed, but alternative splicing of MEF2D transcripts gives rise to a muscle-specific isoform which is induced during myoblast differentiation. The mouse Mef2, Mef2c, and Mef2d genes map to chromosomes 7, 13, and 3, respectively. The complexity of the MEF2 family of regulatory proteins provides the potential for fine-tuning of transcriptional responses as a consequence of combinatorial interactions among multiple MEF2 isoforms encoded by the four Mef2 genes.

Original languageEnglish (US)
Pages (from-to)1647-1656
Number of pages10
JournalMolecular and Cellular Biology
Volume14
Issue number3
StatePublished - Mar 1994

Fingerprint

MEF2 Transcription Factors
Alternative Splicing
Protein Isoforms
Muscles
Messenger RNA
Genes
Chromosomes, Human, Pair 13
Proteins
Chromosomes, Human, Pair 3
Chromosomes, Human, Pair 7
Sequence Deletion
Myoblasts
Transcriptional Activation
Organism Cloning
Transcription Factors

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Martin, J. F., Miano, J. M., Hustad, C. M., Copeland, N. G., Jenkins, N. A., & Olson, E. N. (1994). A Mef2 gene that generates a muscle-specific isoform via alternative mRNA splicing. Molecular and Cellular Biology, 14(3), 1647-1656.

A Mef2 gene that generates a muscle-specific isoform via alternative mRNA splicing. / Martin, James F.; Miano, Joseph M.; Hustad, Carolyn M.; Copeland, Neal G.; Jenkins, Nancy A.; Olson, Eric N.

In: Molecular and Cellular Biology, Vol. 14, No. 3, 03.1994, p. 1647-1656.

Research output: Contribution to journalArticle

Martin, JF, Miano, JM, Hustad, CM, Copeland, NG, Jenkins, NA & Olson, EN 1994, 'A Mef2 gene that generates a muscle-specific isoform via alternative mRNA splicing', Molecular and Cellular Biology, vol. 14, no. 3, pp. 1647-1656.
Martin JF, Miano JM, Hustad CM, Copeland NG, Jenkins NA, Olson EN. A Mef2 gene that generates a muscle-specific isoform via alternative mRNA splicing. Molecular and Cellular Biology. 1994 Mar;14(3):1647-1656.
Martin, James F. ; Miano, Joseph M. ; Hustad, Carolyn M. ; Copeland, Neal G. ; Jenkins, Nancy A. ; Olson, Eric N. / A Mef2 gene that generates a muscle-specific isoform via alternative mRNA splicing. In: Molecular and Cellular Biology. 1994 ; Vol. 14, No. 3. pp. 1647-1656.
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