A modular NIRS system for clinical measurement of impaired skeletal muscle oxygenation

Ramesh Wariar, John N. Gaffke, Ronald G. Haller, Loren A. Bertocci

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Near-infrared spectrometry (NIRS) is a well-known method used to measure in vivo tissue oxygenation and hemodynamics. This method is used to derive relative measures of hemoglobin (Hb) + myoglobin (Mb) oxygenation and total Hb (tHb) accumulation from measurements of optical attenuation at discrete wavelengths. We the design and validation of a new NIRS oxygenation analyzer for the measurement of muscle oxygenation kinetics. This design optimizes optical sensitivity and detector wavelength flexibility while minimizing component and construction costs. Using in vitro validations, we demonstrate 1) general optical linearity, 2) system stability, and 3) measurement accuracy for isolated Hb. Using in vivo validations, we demonstrate 1) expected oxygenation changes during ischemia and reactive hyperemia, 2) expected oxygenation changes during muscle exercise, 3) a close correlation between changes in oxyhemoglobin and oxymyoglobin and changes in deoxyhemoglobin and deoxymyoglobin and limb volume by venous occlusion plethysmography, and 4) a minimal contribution from movement artifact on the detected signals. We also demonstrate the ability of this system to detect abnormal patterns of tissue oxygenation in a well-characterized patient with a deficiency of skeletal muscle coenzyme Q10. We conclude that this is a valid system design for the precise, accurate, and sensitive detection of changes in bulk skeletal muscle oxygenation, can be constructed economically, and can be used diagnostically in patients with disorders of skeletal muscle energy metabolism.

Original languageEnglish (US)
Pages (from-to)315-325
Number of pages11
JournalJournal of Applied Physiology
Volume88
Issue number1
StatePublished - Jan 2000

Fingerprint

Near-Infrared Spectroscopy
Hemoglobins
Skeletal Muscle
coenzyme Q10
Muscles
Oxyhemoglobins
Plethysmography
Myoglobin
Hyperemia
Artifacts
Energy Metabolism
Ischemia
Extremities
Hemodynamics
Exercise
Costs and Cost Analysis

Keywords

  • Coenzyme Q deficiency
  • Exercise
  • Hemoglobin
  • Metabolic disease
  • Movement artifacts
  • Myoglobin
  • Near-infrared spectrometry

ASJC Scopus subject areas

  • Physiology
  • Endocrinology
  • Orthopedics and Sports Medicine
  • Physical Therapy, Sports Therapy and Rehabilitation

Cite this

A modular NIRS system for clinical measurement of impaired skeletal muscle oxygenation. / Wariar, Ramesh; Gaffke, John N.; Haller, Ronald G.; Bertocci, Loren A.

In: Journal of Applied Physiology, Vol. 88, No. 1, 01.2000, p. 315-325.

Research output: Contribution to journalArticle

Wariar, Ramesh ; Gaffke, John N. ; Haller, Ronald G. ; Bertocci, Loren A. / A modular NIRS system for clinical measurement of impaired skeletal muscle oxygenation. In: Journal of Applied Physiology. 2000 ; Vol. 88, No. 1. pp. 315-325.
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