A multiplexed luciferase-based screening platform for interrogating cancer-associated signal transduction in cultured cells.

Ozlem Kulak, Lawrence Lum

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

Genome-scale interrogation of gene function using RNA interference (RNAi) holds tremendous promise for the rapid identification of chemically tractable cancer cell vulnerabilities. Limiting the potential of this technology is the inability to rapidly delineate the mechanistic basis of phenotypic outcomes and thus inform the development of molecularly targeted therapeutic strategies. We outline here methods to deconstruct cellular phenotypes induced by RNAi-mediated gene targeting using multiplexed reporter systems that allow monitoring of key cancer cell-associated processes. This high-content screening methodology is versatile and can be readily adapted for the screening of other types of large molecular libraries.

Original languageEnglish (US)
Pages (from-to)e50369
JournalJournal of visualized experiments : JoVE
Issue number77
DOIs
StatePublished - 2013

ASJC Scopus subject areas

  • Neuroscience(all)
  • Chemical Engineering(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

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