A mutation that prevents GTP-dependent activation of the α chain of Gs

R. Tyler Miller, Susan B. Masters, Kathleen A. Sullivan, Barry Beiderman, Henry R. Bourne

Research output: Contribution to journalArticle

119 Citations (Scopus)

Abstract

Membrane-bound G proteins carry information from receptors on the outside of cells to effector proteins inside cells. The α subunits of these heterotrimeric proteins bind and hydrolyse GTP and control the specificity of interactions with receptor and effector elements1,2. Signalling by G proteins involves a cycle in which the inactive αβγ-GDP complex dissociates to produce α*-GTP, which is capable of activating the effector enzyme or ion channel; the α*-GTP complex hydrolyses bound GTP and reassociates with βγ to form the inactive complex. We have characterized a mutation that interrupts this GTP-driven cycle in αs, the α-chain of Gs, the G protein that stimulates adenylyl cyclase. The mutation converts a glycine to an alanine residue in the presumed GDP-binding domain of αs. The location and biochemical consequences of this mutation suggest a common mechanism by which binding of GTP or ATP may induce changes in the conformation of a number of nucleoside triphosphate binding proteins.

Original languageEnglish (US)
Pages (from-to)712-715
Number of pages4
JournalNature
Volume334
Issue number6184
StatePublished - 1988

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Guanosine Triphosphate
Mutation
GTP-Binding Proteins
Protein Subunits
Ion Channels
Nucleosides
Adenylyl Cyclases
Alanine
Glycine
Carrier Proteins
Adenosine Triphosphate
Membranes
Enzymes
Proteins

ASJC Scopus subject areas

  • General

Cite this

Miller, R. T., Masters, S. B., Sullivan, K. A., Beiderman, B., & Bourne, H. R. (1988). A mutation that prevents GTP-dependent activation of the α chain of Gs Nature, 334(6184), 712-715.

A mutation that prevents GTP-dependent activation of the α chain of Gs . / Miller, R. Tyler; Masters, Susan B.; Sullivan, Kathleen A.; Beiderman, Barry; Bourne, Henry R.

In: Nature, Vol. 334, No. 6184, 1988, p. 712-715.

Research output: Contribution to journalArticle

Miller, RT, Masters, SB, Sullivan, KA, Beiderman, B & Bourne, HR 1988, 'A mutation that prevents GTP-dependent activation of the α chain of Gs ', Nature, vol. 334, no. 6184, pp. 712-715.
Miller RT, Masters SB, Sullivan KA, Beiderman B, Bourne HR. A mutation that prevents GTP-dependent activation of the α chain of Gs Nature. 1988;334(6184):712-715.
Miller, R. Tyler ; Masters, Susan B. ; Sullivan, Kathleen A. ; Beiderman, Barry ; Bourne, Henry R. / A mutation that prevents GTP-dependent activation of the α chain of Gs In: Nature. 1988 ; Vol. 334, No. 6184. pp. 712-715.
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