A new gadolinium-based MRI zinc sensor

Ana C. Esqueda, Jorge A. López, Gabriel Andreu-de-Riquer, José C. Alvarado-Monzón, James Ratnakar, Angelo J M Lubag, A. Dean Sherry, Luis M. De León-Rodríguez

Research output: Contribution to journalArticle

114 Scopus citations

Abstract

The properties of a novel Gd3+-based MRI zinc sensor are reported. Unlike previously reported Gd3+-based MRI contrast agents, this agent (GdL) differs in that the agent alone binds only weakly with human serum albumin (HSA), while the 1:2 GdL:Zn2+ ternary complex binds strongly to HSA resulting in a substantial, 3-fold increase in water proton relaxivity. The GdL complex is shown to have a relatively strong binding affinity for Zn2+ (KD = 33.6 nM), similar to the affinity of the Zn2+ ion with HSA alone. The agent detects as little as 30 μM Zn2+ in the presence of HSA by MRI in vitro, a value slightly more than the total Zn2+ concentration in blood (∼20 μM). This combination of binding affinity constants and the high relaxivity of the agent when bound to HSA suggests that this new agent may be useful for detection of free Zn2+ ions in vivo without disrupting other important biological processes involving Zn2+.

Original languageEnglish (US)
Pages (from-to)11387-11391
Number of pages5
JournalJournal of the American Chemical Society
Volume131
Issue number32
DOIs
StatePublished - Aug 19 2009

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

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    Esqueda, A. C., López, J. A., Andreu-de-Riquer, G., Alvarado-Monzón, J. C., Ratnakar, J., Lubag, A. J. M., Sherry, A. D., & De León-Rodríguez, L. M. (2009). A new gadolinium-based MRI zinc sensor. Journal of the American Chemical Society, 131(32), 11387-11391. https://doi.org/10.1021/ja901875v