A new yeast PUF family protein, Puf6p, represses ASH1 mRNA translation and is required for its localization

Wei Gu, Yingfeng Deng, Daniel Zenklusen, Robert H. Singer

Research output: Contribution to journalArticle

131 Citations (Scopus)

Abstract

In yeast Saccharomyces cerevisiae, Ash1p, a protein determinant for mating-type switching, is segregated within the daughter cell nucleus to establish asymmetry of HO expression. The accumulation of Ash1p results from ASH1 mRNA that is sorted as a ribonucleoprotein particle (mRNP or locasome) to the distal tip of the bud where translation occurs. To study the mechanism regulating ASH1 mRNA translation, we isolated the ASH1 locasome and characterized the associated proteins by MALDI-TOF. One of these proteins was Puf6p, a new member of the PUF family of highly conserved RNA-binding proteins such as Pumilio in Drosophila, responsible for translational repression, usually to effect asymmetric expression. Puf6p-bound PUF consensus sequences in the 3′UTR of ASH1 mRNA and repressed the translation of ASH1 mRNA both in vivo and in vitro. In the puf6A strain, asymmetric localization of both Ash1p and ASH1 mRNA were significantly reduced. We propose that Puf6p is a protein that functions in the translational control of ASH1 mRNA, and this translational inhibition is necessary before localization can proceed.

Original languageEnglish (US)
Pages (from-to)1452-1465
Number of pages14
JournalGenes and Development
Volume18
Issue number12
DOIs
StatePublished - Jun 15 2004

Fingerprint

Protein Biosynthesis
Yeasts
Messenger RNA
Proteins
Ribonucleoproteins
RNA-Binding Proteins
Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Consensus Sequence
Cell Nucleus
Drosophila
Saccharomyces cerevisiae

Keywords

  • ASH1
  • PUF6
  • RNA localization
  • Translational regulation

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology

Cite this

A new yeast PUF family protein, Puf6p, represses ASH1 mRNA translation and is required for its localization. / Gu, Wei; Deng, Yingfeng; Zenklusen, Daniel; Singer, Robert H.

In: Genes and Development, Vol. 18, No. 12, 15.06.2004, p. 1452-1465.

Research output: Contribution to journalArticle

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AU - Deng, Yingfeng

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AU - Singer, Robert H.

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