A novel gene constitutively expressed in human lymphoid cells is inducible with interferon-γ in myeloid cells

Joseph A. Trapani, Kylie A. Browne, Michelle J. Dawson, Robert G. Ramsay, Roger L. Eddy, Thomas B. Shows, Perrin C. White, Bo Dupont

Research output: Contribution to journalArticle

85 Scopus citations

Abstract

A cluster of at lest six interferon-γ (IFNγ)-inducible genes designated Ifi201-204 and located on mouse chromosome 1 has recently been described. Here, we report a human IFN-γ-inducible gene, IFI 16, which has nucleotide sequence similarity with portions of two of the mouse genes, Ifi202 and Ifi204. A full-length cDNA clone derived from IFI 16 [2.709 kilobases (kb)] contained a single open reading frame of 2.187 kb which encoded a putative polypeptide of 729 amino acids and a predicted non-glycosylated Mr of 80020. IFI 16 mRNA was found to be constitutively expressed in lymphoid cells and in cell lines of both the T and B lineages. By contrast, the mRNA was not expresed by the cell lines HL-60, U937, and K562, which represent early stages of myeloid development, but was strongly inducible in HL-60 and U937 with IFN-γ. The IFI 16 protein demonstrated a putative domain structure with patchy similarity to the proteins expressed from gene Ifi202 and Ifi204. The mouse and human proteins each contain two analogous ≈200 amino acid domains which are imperfect copies, but IFI 16 demonstrated additional unique regions, including a Lys-rich N-terminal portion and a "spacer" region between the reiterated domains, analogous to spacer regions in the CD5 and CD8α molecules. Using a panel of inter-species somatic cell hybrid cell lines, IFI 16 was localized to the chromosomal region 1q12→1qter, a region systenic between mouse an man. DNA blotting indicated that, in contrast to the mouse, IFI 16 is present as a single copy gene in the human genome. The authors are pleased to make the cDNA clones described in this paper available to interested investigators.

Original languageEnglish (US)
Pages (from-to)369-376
Number of pages8
JournalImmunogenetics
Volume36
Issue number6
DOIs
StatePublished - Sep 1 1992

ASJC Scopus subject areas

  • Immunology
  • Genetics

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    Trapani, J. A., Browne, K. A., Dawson, M. J., Ramsay, R. G., Eddy, R. L., Shows, T. B., White, P. C., & Dupont, B. (1992). A novel gene constitutively expressed in human lymphoid cells is inducible with interferon-γ in myeloid cells. Immunogenetics, 36(6), 369-376. https://doi.org/10.1007/BF00218044