A novel member of the calcitonin gene-related peptide family, calcitonin receptor-stimulating peptide, inhibits the formation and activity of osteoclasts

Michitaka Notoya, Rumiko Arai, Takeshi Katafuchi, Naoto Minamino, Hiromi Hagiwara

Research output: Contribution to journalArticle

20 Scopus citations

Abstract

We isolated a novel peptide, calcitonin receptor-stimulating peptide-1 (CRSP-1), from porcine brain and found that the administration of this peptide into rats induced a transient decrease in plasma calcium concentration. Therefore, we investigated the effects of CRSP-1 on osteoclastogenesis. Osteoclast-like cells were formed from spleen cells or bone marrow cells by a combination of the receptor activator of nuclear factor-κB ligand (RANKL) and macrophage colony-stimulating factor (M-CSF). CRSP-1 dose-dependently inhibited the formation of multinucleated osteoclast-like cells, and a calcitonin receptor inhibitor antagonized in part the inhibition of osteoclast formation by CRSP-1. Furthermore, CRSP-1 destroyed the actin ring that is a typical index of osteoclast resorption activity; it contributed to this action via the signaling pathway of protein kinase A. Our findings indicate that CRSP-1 inhibits osteoclastogenesis by inhibiting the formation and activity of multinucleated osteoclasts. The inhibitory effects of CRSP-1 on osteoclast metabolism were similar in degree to those of porcine calcitonin. CRSP-1 might provide a clue to the development of tools useful in the prevention and treatment of osteoporosis.

Original languageEnglish (US)
Pages (from-to)234-239
Number of pages6
JournalEuropean Journal of Pharmacology
Volume560
Issue number2-3
DOIs
StatePublished - Apr 10 2007

Keywords

  • Calcitonin
  • Calcitonin receptor-stimulating peptide
  • Osteoclast formation
  • Osteoclasts
  • Protein kinase

ASJC Scopus subject areas

  • Pharmacology

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