A novel non-enzymatic procedure for removing DNA template from RNA transcription mixtures

W. Kedzierski, J. C. Porter

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

A novel, simplified procedure for the removal of DNA template from newly synthesized RNA is described. The method avoids the use of DNase and involves extraction of the RNA transcription products at acid pH using either phenol or phenol: chloroform:guanidinium thiocyanate mixture. Both procedures are as effective as DNase digestion in removing DNA template, but are free of the hazard of RNA degradation, are highly reproducible and remove proteins at the same time.

Original languageEnglish (US)
JournalBioTechniques
Volume10
Issue number2
StatePublished - 1991

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Transcription
Deoxyribonucleases
RNA
Phenol
DNA
RNA Stability
Chloroform
Digestion
Hazards
Degradation
Acids
Proteins

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

A novel non-enzymatic procedure for removing DNA template from RNA transcription mixtures. / Kedzierski, W.; Porter, J. C.

In: BioTechniques, Vol. 10, No. 2, 1991.

Research output: Contribution to journalArticle

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