A novel non-enzymatic procedure for removing DNA template from RNA transcription mixtures

W. Kedzierski; J. C. Porter

A novel non-enzymatic procedure for removing DNA template from RNA transcription mixtures

W. Kedzierski, J. C. Porter

Obstetrics & Gynecology

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56 Scopus citations

Abstract

A novel, simplified procedure for the removal of DNA template from newly synthesized RNA is described. The method avoids the use of DNase and involves extraction of the RNA transcription products at acid pH using either phenol or phenol: chloroform:guanidinium thiocyanate mixture. Both procedures are as effective as DNase digestion in removing DNA template, but are free of the hazard of RNA degradation, are highly reproducible and remove proteins at the same time.

Original language	English (US)
Pages (from-to)	210-212+214
Journal	BioTechniques
Volume	10
Issue number	2
State	Published - 1991

ASJC Scopus subject areas

Biotechnology
General Biochemistry, Genetics and Molecular Biology

Cite this

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title = "A novel non-enzymatic procedure for removing DNA template from RNA transcription mixtures",

abstract = "A novel, simplified procedure for the removal of DNA template from newly synthesized RNA is described. The method avoids the use of DNase and involves extraction of the RNA transcription products at acid pH using either phenol or phenol: chloroform:guanidinium thiocyanate mixture. Both procedures are as effective as DNase digestion in removing DNA template, but are free of the hazard of RNA degradation, are highly reproducible and remove proteins at the same time.",

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AB - A novel, simplified procedure for the removal of DNA template from newly synthesized RNA is described. The method avoids the use of DNase and involves extraction of the RNA transcription products at acid pH using either phenol or phenol: chloroform:guanidinium thiocyanate mixture. Both procedures are as effective as DNase digestion in removing DNA template, but are free of the hazard of RNA degradation, are highly reproducible and remove proteins at the same time.

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A novel non-enzymatic procedure for removing DNA template from RNA transcription mixtures

Abstract

ASJC Scopus subject areas

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