A novel non-enzymatic procedure for removing DNA template from RNA transcription mixtures

W. Kedzierski, J. C. Porter

Research output: Contribution to journalArticlepeer-review

48 Scopus citations

Abstract

A novel, simplified procedure for the removal of DNA template from newly synthesized RNA is described. The method avoids the use of DNase and involves extraction of the RNA transcription products at acid pH using either phenol or phenol: chloroform:guanidinium thiocyanate mixture. Both procedures are as effective as DNase digestion in removing DNA template, but are free of the hazard of RNA degradation, are highly reproducible and remove proteins at the same time.

Original languageEnglish (US)
Pages (from-to)210-212+214
JournalBioTechniques
Volume10
Issue number2
StatePublished - 1991

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry, Genetics and Molecular Biology(all)

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