A phase II study of DT fusion protein denileukin diftitox in patients with fludarabine-refractory chronic lymphocytic leukemia

Arthur E. Frankel, Donald R. Fleming, Philip D. Hall, Bayard L. Powell, Jennifer H. Black, Carole Leftwich, Ronald Gartenhaus

Research output: Contribution to journalArticle

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Abstract

Purpose: Patients with relapsed or refractory chronic lymphocytic leukemia (CLL) have a poor prognosis. We tested the safety and efficacy in these patients of a diphtheria fusion protein DAB389IL2 (denileukin diftitox) directed against the interleukin 2 receptor that is expressed by CLL cells. Experimental Design: DAB389IL2 was administered by 60 min i.v. infusions for 5 days every 21 days at 9 or 18 μg/kg/day for up to eight cycles. Results: Eighteen patients were treated. The mean age of the patients was 61.8 years. There were 14 males and 4 females. Two had Rai stage I, 6 had Rai stage II, and 10 had Rai stage IV. The mean number of prior treatments was 4.5 (range, 1-11). Responses were evaluated by peripheral CLL counts, computed tomography scans of all nodes and bone marrow biopsies. Twelve patients received greater than or equal to three cycles of DAB389IL2 and were evaluable for response. Eleven of 12 patients showed reductions of peripheral CLL cells, with 6 of 11 showing ≥95% reductions. Seven of 12 patients showed reductions of node diameters on exam and computed tomography scans, and 2 of 12 showed 60 and 80% shrinkage, respectively. Pre and postbone marrow biopsies showed a reduction in CLL marrow index in 11 patients. Seven of 11 patients had >50% reduction, including ≥98% reduction in 3 patients. DAB 389IL2 produced 2 of 12 (17%) partial remission and 7 of 12 (58%) minimal responses. Progression-free intervals in the responders were 1, 1, 3+, 4, 9, 10, 10+, 14 and 19+ months. Toxicities were mild to moderate and included asymptomatic, transient transaminasemia, fever, asthenia, hypoalbuminemia, nausea, vomiting, myalgias, rash, anorexia, vascular leak syndrome, and elevated creatinine kinase. Antidiphtheria toxin antibody levels were variable and ranged from 0 to 9 μg/ml (n = 5). Conclusions: DAB389IL2 produced a rapid decrease of leukemic cells in the bone marrow and peripheral blood of most chemotherapy refractory CLL patients. Most patients also tolerated DAB389IL2 well, without significant myelosuppression and/ or immunosuppression. The prolonged progression-free interval and subjectively observed quality-of-life in responders is intriguing. The results suggest DAB389IL2 has biological activity in patients with B-cell CLL. Follow-up studies of combinations or altered schedules or doses to improve the response rate are warranted.

Original languageEnglish (US)
Pages (from-to)3555-3561
Number of pages7
JournalClinical Cancer Research
Volume9
Issue number10 I
StatePublished - Oct 1 2003

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B-Cell Chronic Lymphocytic Leukemia
Proteins
Bone Marrow
denileukin diftitox
fludarabine
Tomography
Biopsy
Asthenia
Hypoalbuminemia
Diphtheria
Interleukin-2 Receptors
Myalgia
Anorexia
Exanthema
Bone Marrow Cells
Immunosuppression
Nausea
Vomiting
Blood Vessels
Creatinine

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Frankel, A. E., Fleming, D. R., Hall, P. D., Powell, B. L., Black, J. H., Leftwich, C., & Gartenhaus, R. (2003). A phase II study of DT fusion protein denileukin diftitox in patients with fludarabine-refractory chronic lymphocytic leukemia. Clinical Cancer Research, 9(10 I), 3555-3561.

A phase II study of DT fusion protein denileukin diftitox in patients with fludarabine-refractory chronic lymphocytic leukemia. / Frankel, Arthur E.; Fleming, Donald R.; Hall, Philip D.; Powell, Bayard L.; Black, Jennifer H.; Leftwich, Carole; Gartenhaus, Ronald.

In: Clinical Cancer Research, Vol. 9, No. 10 I, 01.10.2003, p. 3555-3561.

Research output: Contribution to journalArticle

Frankel, AE, Fleming, DR, Hall, PD, Powell, BL, Black, JH, Leftwich, C & Gartenhaus, R 2003, 'A phase II study of DT fusion protein denileukin diftitox in patients with fludarabine-refractory chronic lymphocytic leukemia', Clinical Cancer Research, vol. 9, no. 10 I, pp. 3555-3561.
Frankel, Arthur E. ; Fleming, Donald R. ; Hall, Philip D. ; Powell, Bayard L. ; Black, Jennifer H. ; Leftwich, Carole ; Gartenhaus, Ronald. / A phase II study of DT fusion protein denileukin diftitox in patients with fludarabine-refractory chronic lymphocytic leukemia. In: Clinical Cancer Research. 2003 ; Vol. 9, No. 10 I. pp. 3555-3561.
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