A phylogenetic survey of biliary lipids in vertebrates

Antonio Moschetta, Fang Xu, Lee R. Hagey, Gerard P. Van Berge-Henegouwen, Karel J. Van Erpecum, Jos F. Brouwers, Jonathan C. Cohen, Molly Bierman, Helen H. Hobbs, Joseph H. Steinbach, Alan F. Hofmann

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Abstract

Biliary lipids (bile salts, phospholipids, cholesterol, plant sterols) were determined in 89 vertebrate species (cartilaginous and bony fish, reptiles, birds, and mammals), and individual phospholipid classes were measured in 35 species. All samples contained conjugated bile salts (C27 bile alcohol sulfates and/or N-acyl amidates of C27 and/or C24 bile acids). Phospholipids were generally absent in the bile of cartilaginous fish and reptiles and were present in low amounts relative to bile salts in bony fish and most birds. In mammals, the phospholipid-bile salt ratio varied widely. The bile from species with low biliary phospholipid-bile salt ratios often contained a high proportion of sphingomyelin, confirmed by HPLC-MS. In species with a high phospholipid-bile salt ratio, the predominant biliary phospholipid was phosphatidylcholine (PC). The phospholipid-bile salt ratio correlated weakly with the calculated weighted hydrophobic index value. Cholesterol was present in the bile of virtually all species, with plant sterols uniformly being present in only trace amounts. The cholesterol-bile salt ratio tended to be higher in mammals than in nonmammals, but bile of all species was unsaturated. Thus, most nonmammalian vertebrates have relatively low levels of biliary phospholipid and cholesterol, suggesting that cholesterol is eliminated predominantly as bile salts. Mammals have a higher phospholipid and cholesterol to bile salt ratio, with the dominant phospholipid being PC.

Original languageEnglish (US)
Pages (from-to)2221-2232
Number of pages12
JournalJournal of Lipid Research
Volume46
Issue number10
DOIs
StatePublished - Oct 2005

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Bile Acids and Salts
Vertebrates
Phospholipids
Lipids
Mammals
Cholesterol
Bile
Fish
Phytosterols
Fishes
Reptiles
Birds
Phosphatidylcholines
Cholestanols
Surveys and Questionnaires
Sphingomyelins
Sulfates
High Pressure Liquid Chromatography

Keywords

  • Bile salts
  • Cholesterol
  • Gas chromatography
  • Mass spectrometry
  • Phosphatidylcholine
  • Sphingomyelin

ASJC Scopus subject areas

  • Endocrinology

Cite this

Moschetta, A., Xu, F., Hagey, L. R., Van Berge-Henegouwen, G. P., Van Erpecum, K. J., Brouwers, J. F., ... Hofmann, A. F. (2005). A phylogenetic survey of biliary lipids in vertebrates. Journal of Lipid Research, 46(10), 2221-2232. https://doi.org/10.1194/jlr.M500178-JLR200

A phylogenetic survey of biliary lipids in vertebrates. / Moschetta, Antonio; Xu, Fang; Hagey, Lee R.; Van Berge-Henegouwen, Gerard P.; Van Erpecum, Karel J.; Brouwers, Jos F.; Cohen, Jonathan C.; Bierman, Molly; Hobbs, Helen H.; Steinbach, Joseph H.; Hofmann, Alan F.

In: Journal of Lipid Research, Vol. 46, No. 10, 10.2005, p. 2221-2232.

Research output: Contribution to journalArticle

Moschetta, A, Xu, F, Hagey, LR, Van Berge-Henegouwen, GP, Van Erpecum, KJ, Brouwers, JF, Cohen, JC, Bierman, M, Hobbs, HH, Steinbach, JH & Hofmann, AF 2005, 'A phylogenetic survey of biliary lipids in vertebrates', Journal of Lipid Research, vol. 46, no. 10, pp. 2221-2232. https://doi.org/10.1194/jlr.M500178-JLR200
Moschetta A, Xu F, Hagey LR, Van Berge-Henegouwen GP, Van Erpecum KJ, Brouwers JF et al. A phylogenetic survey of biliary lipids in vertebrates. Journal of Lipid Research. 2005 Oct;46(10):2221-2232. https://doi.org/10.1194/jlr.M500178-JLR200
Moschetta, Antonio ; Xu, Fang ; Hagey, Lee R. ; Van Berge-Henegouwen, Gerard P. ; Van Erpecum, Karel J. ; Brouwers, Jos F. ; Cohen, Jonathan C. ; Bierman, Molly ; Hobbs, Helen H. ; Steinbach, Joseph H. ; Hofmann, Alan F. / A phylogenetic survey of biliary lipids in vertebrates. In: Journal of Lipid Research. 2005 ; Vol. 46, No. 10. pp. 2221-2232.
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