A post-transcriptional program coordinated by CSDE1 prevents intrinsic neural differentiation of human embryonic stem cells

Hyun Ju Lee, Deniz Bartsch, Cally Xiao, Santiago Guerrero, Gaurav Ahuja, Christina Schindler, James J. Moresco, John R. Yates, Fátima Gebauer, Hisham Bazzi, Christoph Dieterich, Leo Kurian, David Vilchez

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

While the transcriptional network of human embryonic stem cells (hESCs) has been extensively studied, relatively little is known about how post-transcriptional modulations determine hESC function. RNA-binding proteins play central roles in RNA regulation, including translation and turnover. Here we show that the RNA-binding protein CSDE1 (cold shock domain containing E1) is highly expressed in hESCs to maintain their undifferentiated state and prevent default neural fate. Notably, loss of CSDE1 accelerates neural differentiation and potentiates neurogenesis. Conversely, ectopic expression of CSDE1 impairs neural differentiation. We find that CSDE1 post-transcriptionally modulates core components of multiple regulatory nodes of hESC identity, neuroectoderm commitment and neurogenesis. Among these key pro-neural/neuronal factors, CSDE1 binds fatty acid binding protein 7 (FABP7) and vimentin (VIM) mRNAs, as well as transcripts involved in neuron projection development regulating their stability and translation. Thus, our results uncover CSDE1 as a central post-transcriptional regulator of hESC identity and neurogenesis.

Original languageEnglish (US)
Article number1456
JournalNature communications
Volume8
Issue number1
DOIs
StatePublished - Dec 1 2017
Externally publishedYes

Fingerprint

stem cells
Stem cells
Shock
shock
Neurogenesis
RNA-Binding Proteins
proteins
Fatty Acid-Binding Proteins
Neural Plate
Vimentin
Gene Regulatory Networks
regulators
Neurons
fatty acids
neurons
Modulation
Human Embryonic Stem Cells
RNA
projection
Messenger RNA

ASJC Scopus subject areas

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

Cite this

A post-transcriptional program coordinated by CSDE1 prevents intrinsic neural differentiation of human embryonic stem cells. / Ju Lee, Hyun; Bartsch, Deniz; Xiao, Cally; Guerrero, Santiago; Ahuja, Gaurav; Schindler, Christina; Moresco, James J.; Yates, John R.; Gebauer, Fátima; Bazzi, Hisham; Dieterich, Christoph; Kurian, Leo; Vilchez, David.

In: Nature communications, Vol. 8, No. 1, 1456, 01.12.2017.

Research output: Contribution to journalArticle

Ju Lee, H, Bartsch, D, Xiao, C, Guerrero, S, Ahuja, G, Schindler, C, Moresco, JJ, Yates, JR, Gebauer, F, Bazzi, H, Dieterich, C, Kurian, L & Vilchez, D 2017, 'A post-transcriptional program coordinated by CSDE1 prevents intrinsic neural differentiation of human embryonic stem cells', Nature communications, vol. 8, no. 1, 1456. https://doi.org/10.1038/s41467-017-01744-5
Ju Lee, Hyun ; Bartsch, Deniz ; Xiao, Cally ; Guerrero, Santiago ; Ahuja, Gaurav ; Schindler, Christina ; Moresco, James J. ; Yates, John R. ; Gebauer, Fátima ; Bazzi, Hisham ; Dieterich, Christoph ; Kurian, Leo ; Vilchez, David. / A post-transcriptional program coordinated by CSDE1 prevents intrinsic neural differentiation of human embryonic stem cells. In: Nature communications. 2017 ; Vol. 8, No. 1.
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