A potential mechanism of local anti-inflammatory action of alpha- melanocyte-stimulating hormone within the brain: Modulation of tumor necrosis factor-alpha production by human astrocytic cells

Kit Yue Wong, Nilum Rajora, Giovanni Boccoli, Anna Catania, James M. Lipton

Research output: Contribution to journalArticle

59 Citations (Scopus)

Abstract

The proinflammatory cytokine tumor necrosis factor-α (TNF-α) occurs in CNS tissue in neurological disorders, infection, and injury. Its excessive production is believed to contribute to local pathology, in which case modulation of TNF-α production should promote survival of neural tissue. The neuropeptide α-melanocyte stimulating hormone [α-MSH (1-13)] inhibits TNF- α production in vivo and in vitro, and in this research we tested the capacity of the peptide, and of an anti-inflammatory COOH-terminal tripeptide fragment of it, to inhibit TNF-α production induced by bacterial endotoxin in cells of a human glioma line (A-172, anaplastic astrocytoma cells). Both peptides were effective, although the α-MSH (1-13) sequence was more potent. Preincubation of the cells with α-MSH (1-13) markedly increased its effectiveness. The anticytokine effect of α-MSH in glioma cells may be mediated by human melanocortin-1 receptors; mRNA for this receptor subtype was isolated from resting A-172 cells. These results, combined with prior evidence of effectiveness of α-MSH molecules in modulating inflammatory processes and of their low toxicity, suggest that the molecules may be useful in the treatment of CNS disorders that have an inflammatory component.

Original languageEnglish (US)
Pages (from-to)37-41
Number of pages5
JournalNeuroImmunoModulation
Volume4
Issue number1
StatePublished - Sep 1997

Fingerprint

Melanocyte-Stimulating Hormones
alpha-MSH
Brain Neoplasms
Anti-Inflammatory Agents
Tumor Necrosis Factor-alpha
Glioma
Receptor, Melanocortin, Type 1
Tissue Survival
Peptides
Astrocytoma
Nervous System Diseases
Neuropeptides
Endotoxins
Pathology
Cytokines
Messenger RNA
Wounds and Injuries
Infection
Research

Keywords

  • α-Melanocyte-stimulating hormone
  • CNS disorders
  • Inflammation
  • Neurological disorders
  • Tumor necrosis factor-α

ASJC Scopus subject areas

  • Endocrinology
  • Neuroscience(all)
  • Immunology

Cite this

A potential mechanism of local anti-inflammatory action of alpha- melanocyte-stimulating hormone within the brain : Modulation of tumor necrosis factor-alpha production by human astrocytic cells. / Wong, Kit Yue; Rajora, Nilum; Boccoli, Giovanni; Catania, Anna; Lipton, James M.

In: NeuroImmunoModulation, Vol. 4, No. 1, 09.1997, p. 37-41.

Research output: Contribution to journalArticle

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