A rapid method to assess the hydrophobicity of the intestinal microvillus membrane in vivo

J. B. Meddings, J. M. Dietschy

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

Absorption rates for many biologically important compounds are determined by the relative hydrophobicity of the jejunal microvillus membrane. An estimate of this parameter may be obtained by measuring the incremental change in free energy that occurs when a methylene group partitions into the bilayer from an external aqueous solution. Although sensitive, this measurement has been difficult to quantitate in vivo; therefore, these studies have historically been performed in vitro. We describe a rapid, simple technique to measure this parameter in vivo. Furthermore, this method directly quantitates the resistance of aqueous unstirred layers that lie external to the microvillus membrane.

Original languageEnglish (US)
Pages (from-to)1289-1296
Number of pages8
JournalJournal of lipid research
Volume30
Issue number8
StatePublished - Jan 1 1989

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Cell Biology

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