A rapid method to assess the hydrophobicity of the intestinal microvillus membrane in vivo

J. B. Meddings, J. M. Dietschy

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4 Scopus citations


Absorption rates for many biologically important compounds are determined by the relative hydrophobicity of the jejunal microvillus membrane. An estimate of this parameter may be obtained by measuring the incremental change in free energy that occurs when a methylene group partitions into the bilayer from an external aqueous solution. Although sensitive, this measurement has been difficult to quantitate in vivo; therefore, these studies have historically been performed in vitro. We describe a rapid, simple technique to measure this parameter in vivo. Furthermore, this method directly quantitates the resistance of aqueous unstirred layers that lie external to the microvillus membrane.

Original languageEnglish (US)
Pages (from-to)1289-1296
Number of pages8
JournalJournal of Lipid Research
Issue number8
Publication statusPublished - 1989


ASJC Scopus subject areas

  • Endocrinology

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