TY - JOUR
T1 - A rapid, multiwell colorimetric assay for chemotaxis
AU - Shi, Yufang
AU - Kornovski, Barbara S.
AU - Savani, Rashmin
AU - Turley, Eva A.
PY - 1993/9/15
Y1 - 1993/9/15
N2 - This paper describes a colorimetric assay for the rapid quantification of chemotaxis in multiple samples. In this assay, cells that have migrated through polycarbonate membrane filters are collected onto the bottom wells of a chemotaxis chamber after centrifugation then the number of viable cells collected in the bottom well is quantified by measurement of the reduction of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenol tetrazolium bromide (MTT). The degree of MTT reduction, which corresponds to the relative cell number, is measured automatically with an ELISA reader. The MTT method of quantitation is as sensitive as the standard manual method, is especially useful for large numbers of samples and requires no specialized laboratory equipment.
AB - This paper describes a colorimetric assay for the rapid quantification of chemotaxis in multiple samples. In this assay, cells that have migrated through polycarbonate membrane filters are collected onto the bottom wells of a chemotaxis chamber after centrifugation then the number of viable cells collected in the bottom well is quantified by measurement of the reduction of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenol tetrazolium bromide (MTT). The degree of MTT reduction, which corresponds to the relative cell number, is measured automatically with an ELISA reader. The MTT method of quantitation is as sensitive as the standard manual method, is especially useful for large numbers of samples and requires no specialized laboratory equipment.
KW - Chemotaxis
KW - Colorimetric assay
KW - MTT
UR - http://www.scopus.com/inward/record.url?scp=0027435391&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0027435391&partnerID=8YFLogxK
U2 - 10.1016/0022-1759(93)90307-S
DO - 10.1016/0022-1759(93)90307-S
M3 - Article
C2 - 8370922
AN - SCOPUS:0027435391
SN - 0022-1759
VL - 164
SP - 149
EP - 154
JO - Journal of Immunological Methods
JF - Journal of Immunological Methods
IS - 2
ER -