A rapid, multiwell colorimetric assay for chemotaxis

Yufang Shi, Barbara S. Kornovski, Rashmin Savani, Eva A. Turley

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

This paper describes a colorimetric assay for the rapid quantification of chemotaxis in multiple samples. In this assay, cells that have migrated through polycarbonate membrane filters are collected onto the bottom wells of a chemotaxis chamber after centrifugation then the number of viable cells collected in the bottom well is quantified by measurement of the reduction of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenol tetrazolium bromide (MTT). The degree of MTT reduction, which corresponds to the relative cell number, is measured automatically with an ELISA reader. The MTT method of quantitation is as sensitive as the standard manual method, is especially useful for large numbers of samples and requires no specialized laboratory equipment.

Original languageEnglish (US)
Pages (from-to)149-154
Number of pages6
JournalJournal of Immunological Methods
Volume164
Issue number2
DOIs
StatePublished - Sep 15 1993

Fingerprint

polycarbonate
Chemotaxis
Cell Count
Bromides
Centrifugation
Enzyme-Linked Immunosorbent Assay
Equipment and Supplies
Membranes
thiazolyl blue

Keywords

  • Chemotaxis
  • Colorimetric assay
  • MTT

ASJC Scopus subject areas

  • Biotechnology
  • Immunology

Cite this

A rapid, multiwell colorimetric assay for chemotaxis. / Shi, Yufang; Kornovski, Barbara S.; Savani, Rashmin; Turley, Eva A.

In: Journal of Immunological Methods, Vol. 164, No. 2, 15.09.1993, p. 149-154.

Research output: Contribution to journalArticle

Shi, Yufang ; Kornovski, Barbara S. ; Savani, Rashmin ; Turley, Eva A. / A rapid, multiwell colorimetric assay for chemotaxis. In: Journal of Immunological Methods. 1993 ; Vol. 164, No. 2. pp. 149-154.
@article{33a58e7a25534ac8a1fbcd1f6be756e9,
title = "A rapid, multiwell colorimetric assay for chemotaxis",
abstract = "This paper describes a colorimetric assay for the rapid quantification of chemotaxis in multiple samples. In this assay, cells that have migrated through polycarbonate membrane filters are collected onto the bottom wells of a chemotaxis chamber after centrifugation then the number of viable cells collected in the bottom well is quantified by measurement of the reduction of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenol tetrazolium bromide (MTT). The degree of MTT reduction, which corresponds to the relative cell number, is measured automatically with an ELISA reader. The MTT method of quantitation is as sensitive as the standard manual method, is especially useful for large numbers of samples and requires no specialized laboratory equipment.",
keywords = "Chemotaxis, Colorimetric assay, MTT",
author = "Yufang Shi and Kornovski, {Barbara S.} and Rashmin Savani and Turley, {Eva A.}",
year = "1993",
month = "9",
day = "15",
doi = "10.1016/0022-1759(93)90307-S",
language = "English (US)",
volume = "164",
pages = "149--154",
journal = "Journal of Immunological Methods",
issn = "0022-1759",
publisher = "Elsevier",
number = "2",

}

TY - JOUR

T1 - A rapid, multiwell colorimetric assay for chemotaxis

AU - Shi, Yufang

AU - Kornovski, Barbara S.

AU - Savani, Rashmin

AU - Turley, Eva A.

PY - 1993/9/15

Y1 - 1993/9/15

N2 - This paper describes a colorimetric assay for the rapid quantification of chemotaxis in multiple samples. In this assay, cells that have migrated through polycarbonate membrane filters are collected onto the bottom wells of a chemotaxis chamber after centrifugation then the number of viable cells collected in the bottom well is quantified by measurement of the reduction of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenol tetrazolium bromide (MTT). The degree of MTT reduction, which corresponds to the relative cell number, is measured automatically with an ELISA reader. The MTT method of quantitation is as sensitive as the standard manual method, is especially useful for large numbers of samples and requires no specialized laboratory equipment.

AB - This paper describes a colorimetric assay for the rapid quantification of chemotaxis in multiple samples. In this assay, cells that have migrated through polycarbonate membrane filters are collected onto the bottom wells of a chemotaxis chamber after centrifugation then the number of viable cells collected in the bottom well is quantified by measurement of the reduction of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenol tetrazolium bromide (MTT). The degree of MTT reduction, which corresponds to the relative cell number, is measured automatically with an ELISA reader. The MTT method of quantitation is as sensitive as the standard manual method, is especially useful for large numbers of samples and requires no specialized laboratory equipment.

KW - Chemotaxis

KW - Colorimetric assay

KW - MTT

UR - http://www.scopus.com/inward/record.url?scp=0027435391&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027435391&partnerID=8YFLogxK

U2 - 10.1016/0022-1759(93)90307-S

DO - 10.1016/0022-1759(93)90307-S

M3 - Article

C2 - 8370922

AN - SCOPUS:0027435391

VL - 164

SP - 149

EP - 154

JO - Journal of Immunological Methods

JF - Journal of Immunological Methods

SN - 0022-1759

IS - 2

ER -