A rapid whole-mount staining procedure for nuclei of mammalian embryos

V. G. Pursel, R. J. Wall, C. E. Rexroad, Robert E Hammer, R. L. Brinster

Research output: Contribution to journalArticle

147 Scopus citations

Abstract

A rapid staining procedure for examining nuclei of mammalian embryos is described. Embryos are placed on a glass slide, counterstained with trypan blue, stained with Hoechst 33342, and embedded between the slide and coverslip in Permount. The nuclei of the embryos fluoresce brightly when examined by fluorescence microscopy immediately after staining or after extended storage. The technique has proved to be an effective tool for studying the development of cow, hamster, mouse, pig, rabbit, and sheep embryos.

Original languageEnglish (US)
Pages (from-to)687-691
Number of pages5
JournalTheriogenology
Volume24
Issue number6
DOIs
StatePublished - Dec 1985

Keywords

  • Hoechst 33342
  • embryos
  • fluorescence
  • nuclear stain
  • trypan blue

ASJC Scopus subject areas

  • Small Animals
  • Food Animals
  • Animal Science and Zoology
  • Equine

Fingerprint Dive into the research topics of 'A rapid whole-mount staining procedure for nuclei of mammalian embryos'. Together they form a unique fingerprint.

  • Cite this