A role for cofilin and LIM kinase in Listeria-induced phagocytosis

Hélène Bierne, Edith Gouin, Pascal Roux, Pico Caroni, Helen L. Yin, Pascale Cossart

Research output: Contribution to journalArticle

135 Citations (Scopus)

Abstract

The pathogenic bacterium Listeria monocytogenes is able to invade nonphagocytic cells, an essential feature for its pathogenicity. This induced phagocytosis process requires tightly regulated steps of actin polymerization and depolymerization. Here, we investigated how interactions of the invasion protein InlB with mammalian cells control the cytoskeleton during Listeria internalization. By fluorescence microscopy and transfection experiments, we show that the actin-nucleating Arp2/3 complex, the GTPase Rac, LIM kinase (LIMK), and cofilin are key proteins in InlB-induced phagocytosis. Overexpression of LIMK1, which has been shown to phosphorylate and inactivate cofilin, induces accumulation of F-actin beneath entering particles and inhibits internalization. Conversely, inhibition of LIMK's activity by expressing a dominant negative construct, LIMK1-, or expression of the constitutively active S3A cofilin mutant induces loss of actin filaments at the phagocytic cup and also inhibits phagocytosis. Interestingly, those constructs similarly affect other actin-based phenomenons, such as InlB-induced membrane ruffling or Listeria comet tail formations. Thus, our data provide evidence for a control of phagocytosis by both activation and deactivation of cofilin. We propose a model in which cofilin is involved in the formation and disruption of the phagocytic cup as a result of its local progressive enrichment.

Original languageEnglish (US)
Pages (from-to)101-112
Number of pages12
JournalJournal of Cell Biology
Volume155
Issue number1
DOIs
StatePublished - Oct 1 2001

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Lim Kinases
Actin Depolymerizing Factors
Listeria
Phagocytosis
Actins
Actin-Related Protein 2-3 Complex
GTP Phosphohydrolases
Listeria monocytogenes
Cytoskeleton
Actin Cytoskeleton
Fluorescence Microscopy
Polymerization
Transfection
Virulence
Bacteria
Membranes

Keywords

  • Actin-based
  • Cyroskeleton
  • InlB
  • Listeria monocytogenes
  • Met
  • Motility

ASJC Scopus subject areas

  • Cell Biology

Cite this

A role for cofilin and LIM kinase in Listeria-induced phagocytosis. / Bierne, Hélène; Gouin, Edith; Roux, Pascal; Caroni, Pico; Yin, Helen L.; Cossart, Pascale.

In: Journal of Cell Biology, Vol. 155, No. 1, 01.10.2001, p. 101-112.

Research output: Contribution to journalArticle

Bierne, H, Gouin, E, Roux, P, Caroni, P, Yin, HL & Cossart, P 2001, 'A role for cofilin and LIM kinase in Listeria-induced phagocytosis', Journal of Cell Biology, vol. 155, no. 1, pp. 101-112. https://doi.org/10.1083/jcb.200104037
Bierne, Hélène ; Gouin, Edith ; Roux, Pascal ; Caroni, Pico ; Yin, Helen L. ; Cossart, Pascale. / A role for cofilin and LIM kinase in Listeria-induced phagocytosis. In: Journal of Cell Biology. 2001 ; Vol. 155, No. 1. pp. 101-112.
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