A single gene encodes soluble and membrane-bound forms of the major histocompatibility Qa-2 antigen: Anchoring of the product by a phospholipid tail

Iwona Stroynowski, Mark Soloski, Martin G. Low, Leroy Hood

Research output: Contribution to journalArticle

85 Scopus citations

Abstract

The H-2, Qa, and Tla genes of the murine major histocompatibility complex are related to each other by DNA sequence homology. The H-2 genes encode ubiquitously expressed transplantation antigens that serve as recognition structures for cytotoxic T cells. The identities of the Qa and Tla products, their sites of expression, and their functions are largely unknown. We report here that the Qa region gene Q7 encodes a membrane-bound as well as a secreted form of the serologically defined antigen Qa-2. The Q7 gene introduced into liver-derived cells is expressed as a membrane-bound and as a secreted molecule. In transfected L cells it is expressed only as a soluble protein. Biochemical analysis suggests that the Q7 product is anchored to the liver cell membranes by a phospholipid tail. This feature may be responsible for cell type-specific expression of the two forms of the Qa-2 molecules.

Original languageEnglish (US)
Pages (from-to)759-768
Number of pages10
JournalCell
Volume50
Issue number5
DOIs
StatePublished - Aug 28 1987

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Fingerprint Dive into the research topics of 'A single gene encodes soluble and membrane-bound forms of the major histocompatibility Qa-2 antigen: Anchoring of the product by a phospholipid tail'. Together they form a unique fingerprint.

  • Cite this