A study of adenosine 3′:5′-cyclic monophosphate, sodium butyrate and cortisol as inducers of HeLa alkaline phosphatase

M. J. Griffin, G. H. Price, K. L. Bazzell, R. P. Cox, N. K. Ghosh

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Abstract

The role of adenosine 3′:5′-cyclic monophosphate in the cortisol-mediated induction of HeLa 65 alkaline phosphatase was investigated. Although growth of these cells with 0.5-1.0 mm N6,O2′-dibutyryl adenosine 3′:5′-cyclic monophosphate induces a 5- to 8-fold increase in cellular phosphatase activity after 72 hr, neither cAMP nor theophylline induce at concentrations up to 1 mm. Sodium butyrate induces the enzyme as well as dibutyryl cAMP. Moreover, induction kinetics show sodium butyrate to be a more efficient inducer than dibutyryl cAMP, inducing activity as quickly as cortisol. This suggests that the butyric acid cleaved from dibutyryl cAMP by HeLa cells is the mediator of induction when the cyclic nucleotide derivative is used.

Original languageEnglish (US)
Pages (from-to)619-623
Number of pages5
JournalArchives of Biochemistry and Biophysics
Volume164
Issue number2
DOIs
StatePublished - Oct 1974

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ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology

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