A YAC Contig of Approximately 3 Mb from Human Chromosome 5q31 → q33

Xiang Li, Carol A. Wise, Denis Le Paslier, Anita L. Hawkins, Constance A. Griffin, Steven J. Pittler, Michael Lovett, Ethylin Wang Jabs

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

The human chromosome 5q31-q33 region contains an interesting cluster of growth factor and receptor genes. In addition, several genetic disease loci have been localized within this region, but have not as yet been isolated as molecular clones. These include those loci involved in autosomal dominant limb-girdle muscular dystrophy, diastrophic dysplasia. Treacher Collins syndrome, and myeloid disorders associated with the 5q- syndrome. A yeast artificial chromosome (YAC) contig of this region would assist in the further localization and isolation of these genes. We have used YACs isolated from the Washington University and Centre d'Etude du Polymorphisme Humain YAC libraries, including YACs from the large insert (mega) YAC library to build a contig greater than 3 Mb in size. An STS content strategy coupled with limited walking from YAC ends was used to isolate 22 overlapping YACs with as much as sixfold coverage. A total of 20 STSs, derived from genes, anonymous sequences, and vector Alu-PCR or inverse PCR products, were used to compile this contig. The order of loci, centromere-GRL-D5S207-D5S70-D5S545-D5S546-D5S547-D5S68-D5S548-D5S210-D5S 549-D5S686-ADRB2-D5S559-CSF1R-D5S551-RPS14-D5S519-SPARC-telomere, was derived from the overlapping clones. This contig and clones derived from it will be useful substrates in selecting candidate cDNAs for the disease loci in this interval.

Original languageEnglish (US)
Pages (from-to)470-477
Number of pages8
JournalGenomics
Volume19
Issue number3
DOIs
StatePublished - Feb 1994

Fingerprint

Yeast Artificial Chromosomes
Human Chromosomes
Clone Cells
Mandibulofacial Dysostosis
Limb-Girdle Muscular Dystrophies
Genes
Polymerase Chain Reaction
Inborn Genetic Diseases
Genetic Loci
Growth Factor Receptors
Centromere
Telomere
Walking
Complementary DNA

ASJC Scopus subject areas

  • Genetics

Cite this

Li, X., Wise, C. A., Le Paslier, D., Hawkins, A. L., Griffin, C. A., Pittler, S. J., ... Jabs, E. W. (1994). A YAC Contig of Approximately 3 Mb from Human Chromosome 5q31 → q33. Genomics, 19(3), 470-477. https://doi.org/10.1006/geno.1994.1096

A YAC Contig of Approximately 3 Mb from Human Chromosome 5q31 → q33. / Li, Xiang; Wise, Carol A.; Le Paslier, Denis; Hawkins, Anita L.; Griffin, Constance A.; Pittler, Steven J.; Lovett, Michael; Jabs, Ethylin Wang.

In: Genomics, Vol. 19, No. 3, 02.1994, p. 470-477.

Research output: Contribution to journalArticle

Li, X, Wise, CA, Le Paslier, D, Hawkins, AL, Griffin, CA, Pittler, SJ, Lovett, M & Jabs, EW 1994, 'A YAC Contig of Approximately 3 Mb from Human Chromosome 5q31 → q33', Genomics, vol. 19, no. 3, pp. 470-477. https://doi.org/10.1006/geno.1994.1096
Li X, Wise CA, Le Paslier D, Hawkins AL, Griffin CA, Pittler SJ et al. A YAC Contig of Approximately 3 Mb from Human Chromosome 5q31 → q33. Genomics. 1994 Feb;19(3):470-477. https://doi.org/10.1006/geno.1994.1096
Li, Xiang ; Wise, Carol A. ; Le Paslier, Denis ; Hawkins, Anita L. ; Griffin, Constance A. ; Pittler, Steven J. ; Lovett, Michael ; Jabs, Ethylin Wang. / A YAC Contig of Approximately 3 Mb from Human Chromosome 5q31 → q33. In: Genomics. 1994 ; Vol. 19, No. 3. pp. 470-477.
@article{0751f23ff199430387067e4c875dc540,
title = "A YAC Contig of Approximately 3 Mb from Human Chromosome 5q31 → q33",
abstract = "The human chromosome 5q31-q33 region contains an interesting cluster of growth factor and receptor genes. In addition, several genetic disease loci have been localized within this region, but have not as yet been isolated as molecular clones. These include those loci involved in autosomal dominant limb-girdle muscular dystrophy, diastrophic dysplasia. Treacher Collins syndrome, and myeloid disorders associated with the 5q- syndrome. A yeast artificial chromosome (YAC) contig of this region would assist in the further localization and isolation of these genes. We have used YACs isolated from the Washington University and Centre d'Etude du Polymorphisme Humain YAC libraries, including YACs from the large insert (mega) YAC library to build a contig greater than 3 Mb in size. An STS content strategy coupled with limited walking from YAC ends was used to isolate 22 overlapping YACs with as much as sixfold coverage. A total of 20 STSs, derived from genes, anonymous sequences, and vector Alu-PCR or inverse PCR products, were used to compile this contig. The order of loci, centromere-GRL-D5S207-D5S70-D5S545-D5S546-D5S547-D5S68-D5S548-D5S210-D5S 549-D5S686-ADRB2-D5S559-CSF1R-D5S551-RPS14-D5S519-SPARC-telomere, was derived from the overlapping clones. This contig and clones derived from it will be useful substrates in selecting candidate cDNAs for the disease loci in this interval.",
author = "Xiang Li and Wise, {Carol A.} and {Le Paslier}, Denis and Hawkins, {Anita L.} and Griffin, {Constance A.} and Pittler, {Steven J.} and Michael Lovett and Jabs, {Ethylin Wang}",
year = "1994",
month = "2",
doi = "10.1006/geno.1994.1096",
language = "English (US)",
volume = "19",
pages = "470--477",
journal = "Genomics",
issn = "0888-7543",
publisher = "Academic Press Inc.",
number = "3",

}

TY - JOUR

T1 - A YAC Contig of Approximately 3 Mb from Human Chromosome 5q31 → q33

AU - Li, Xiang

AU - Wise, Carol A.

AU - Le Paslier, Denis

AU - Hawkins, Anita L.

AU - Griffin, Constance A.

AU - Pittler, Steven J.

AU - Lovett, Michael

AU - Jabs, Ethylin Wang

PY - 1994/2

Y1 - 1994/2

N2 - The human chromosome 5q31-q33 region contains an interesting cluster of growth factor and receptor genes. In addition, several genetic disease loci have been localized within this region, but have not as yet been isolated as molecular clones. These include those loci involved in autosomal dominant limb-girdle muscular dystrophy, diastrophic dysplasia. Treacher Collins syndrome, and myeloid disorders associated with the 5q- syndrome. A yeast artificial chromosome (YAC) contig of this region would assist in the further localization and isolation of these genes. We have used YACs isolated from the Washington University and Centre d'Etude du Polymorphisme Humain YAC libraries, including YACs from the large insert (mega) YAC library to build a contig greater than 3 Mb in size. An STS content strategy coupled with limited walking from YAC ends was used to isolate 22 overlapping YACs with as much as sixfold coverage. A total of 20 STSs, derived from genes, anonymous sequences, and vector Alu-PCR or inverse PCR products, were used to compile this contig. The order of loci, centromere-GRL-D5S207-D5S70-D5S545-D5S546-D5S547-D5S68-D5S548-D5S210-D5S 549-D5S686-ADRB2-D5S559-CSF1R-D5S551-RPS14-D5S519-SPARC-telomere, was derived from the overlapping clones. This contig and clones derived from it will be useful substrates in selecting candidate cDNAs for the disease loci in this interval.

AB - The human chromosome 5q31-q33 region contains an interesting cluster of growth factor and receptor genes. In addition, several genetic disease loci have been localized within this region, but have not as yet been isolated as molecular clones. These include those loci involved in autosomal dominant limb-girdle muscular dystrophy, diastrophic dysplasia. Treacher Collins syndrome, and myeloid disorders associated with the 5q- syndrome. A yeast artificial chromosome (YAC) contig of this region would assist in the further localization and isolation of these genes. We have used YACs isolated from the Washington University and Centre d'Etude du Polymorphisme Humain YAC libraries, including YACs from the large insert (mega) YAC library to build a contig greater than 3 Mb in size. An STS content strategy coupled with limited walking from YAC ends was used to isolate 22 overlapping YACs with as much as sixfold coverage. A total of 20 STSs, derived from genes, anonymous sequences, and vector Alu-PCR or inverse PCR products, were used to compile this contig. The order of loci, centromere-GRL-D5S207-D5S70-D5S545-D5S546-D5S547-D5S68-D5S548-D5S210-D5S 549-D5S686-ADRB2-D5S559-CSF1R-D5S551-RPS14-D5S519-SPARC-telomere, was derived from the overlapping clones. This contig and clones derived from it will be useful substrates in selecting candidate cDNAs for the disease loci in this interval.

UR - http://www.scopus.com/inward/record.url?scp=0028288409&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028288409&partnerID=8YFLogxK

U2 - 10.1006/geno.1994.1096

DO - 10.1006/geno.1994.1096

M3 - Article

C2 - 8188289

AN - SCOPUS:0028288409

VL - 19

SP - 470

EP - 477

JO - Genomics

JF - Genomics

SN - 0888-7543

IS - 3

ER -