Abnormal expression of BRCA1 and BRCA1-interactive DNA-repair proteins in breast carcinomas

Kiyotsugu Yoshikawa, Tomoko Ogawa, Richard Baer, Hiromichi Hemmi, Kazuo Honda, Akira Yamauchi, Takashi Inamoto, Kohaku Ko, Shujiro Yazumi, Hirotoshi Motoda, Hiroshi Kodama, Shinzaburo Noguchi, Adi F. Gazdar, Yoshio Yamaoka, Rei Takahashi

Research output: Contribution to journalArticle

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Abstract

Breast cancer is one of the most common malignancies among women. The molecular mechanisms involved in breast carcinogenesis, however, remain to be elucidated. Although somatic mutation of BRCA1 is rare, BRCA1 protein expression is reduced in about 30% of sporadic breast carcinomas (Yoshikawa et al., Clin. Cancer Res., 5:1249-1261, 1999), indicating its possible involvement even in sporadic breast carcinogenesis. Among the BRCA1-interactive proteins are hRAD51 (a human homologue of Escherichia coli rec A protein), BARD1 (BRCA1-associated RING domain 1) and p53, all of which are involved in DNA repair. We have analyzed the expression patterns of the hRAD51, BARD1 and p53 proteins in five breast cancer cell lines, including a BRCA1-deficient cell line, and in 179 breast cancer tissue samples from Japanese women, including 113 sporadic, 47 hereditary (i.e., BRCA1 status unknown), and 19 BRCA1-associated cases. Of the 179 breast carcinomas, fifty-four (30%) exhibited reduced hRAD51 expression, and sixty-two (35%) exhibited p53 overexpression. On the other hand, reduced expression level of BARD1, and of hMSH2 and hMLH1, which are components of DNA mismatch-repair pathway and are involved in colorectal carcinogenesis, was observed respectively in only 10 (6%), 8 (5%) and 3 (2%) cases. The overall frequency of sporadic breast carcinomas with abnormal expression of either BRCA1 or the BRCA1-interactive proteins was 67% (76/113). These results indicate that there may be an important role for the BRCA1-associated DNA-repair pathway, not only in BRCA1-associated breast carcinomas, but also in sporadic breast carcinomas. (C) 2000 Wiley-Liss, Inc.

Original languageEnglish (US)
Pages (from-to)28-36
Number of pages9
JournalInternational Journal of Cancer
Volume88
Issue number1
DOIs
StatePublished - 2000

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DNA Repair
Breast Neoplasms
BRCA1 Protein
Proteins
Carcinogenesis
Breast
Rec A Recombinases
Cell Line
DNA Mismatch Repair
Neoplasms
Escherichia coli
Mutation

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Abnormal expression of BRCA1 and BRCA1-interactive DNA-repair proteins in breast carcinomas. / Yoshikawa, Kiyotsugu; Ogawa, Tomoko; Baer, Richard; Hemmi, Hiromichi; Honda, Kazuo; Yamauchi, Akira; Inamoto, Takashi; Ko, Kohaku; Yazumi, Shujiro; Motoda, Hirotoshi; Kodama, Hiroshi; Noguchi, Shinzaburo; Gazdar, Adi F.; Yamaoka, Yoshio; Takahashi, Rei.

In: International Journal of Cancer, Vol. 88, No. 1, 2000, p. 28-36.

Research output: Contribution to journalArticle

Yoshikawa, K, Ogawa, T, Baer, R, Hemmi, H, Honda, K, Yamauchi, A, Inamoto, T, Ko, K, Yazumi, S, Motoda, H, Kodama, H, Noguchi, S, Gazdar, AF, Yamaoka, Y & Takahashi, R 2000, 'Abnormal expression of BRCA1 and BRCA1-interactive DNA-repair proteins in breast carcinomas', International Journal of Cancer, vol. 88, no. 1, pp. 28-36. https://doi.org/10.1002/1097-0215(20001001)88:1<28::AID-IJC5>3.0.CO;2-4
Yoshikawa, Kiyotsugu ; Ogawa, Tomoko ; Baer, Richard ; Hemmi, Hiromichi ; Honda, Kazuo ; Yamauchi, Akira ; Inamoto, Takashi ; Ko, Kohaku ; Yazumi, Shujiro ; Motoda, Hirotoshi ; Kodama, Hiroshi ; Noguchi, Shinzaburo ; Gazdar, Adi F. ; Yamaoka, Yoshio ; Takahashi, Rei. / Abnormal expression of BRCA1 and BRCA1-interactive DNA-repair proteins in breast carcinomas. In: International Journal of Cancer. 2000 ; Vol. 88, No. 1. pp. 28-36.
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abstract = "Breast cancer is one of the most common malignancies among women. The molecular mechanisms involved in breast carcinogenesis, however, remain to be elucidated. Although somatic mutation of BRCA1 is rare, BRCA1 protein expression is reduced in about 30{\%} of sporadic breast carcinomas (Yoshikawa et al., Clin. Cancer Res., 5:1249-1261, 1999), indicating its possible involvement even in sporadic breast carcinogenesis. Among the BRCA1-interactive proteins are hRAD51 (a human homologue of Escherichia coli rec A protein), BARD1 (BRCA1-associated RING domain 1) and p53, all of which are involved in DNA repair. We have analyzed the expression patterns of the hRAD51, BARD1 and p53 proteins in five breast cancer cell lines, including a BRCA1-deficient cell line, and in 179 breast cancer tissue samples from Japanese women, including 113 sporadic, 47 hereditary (i.e., BRCA1 status unknown), and 19 BRCA1-associated cases. Of the 179 breast carcinomas, fifty-four (30{\%}) exhibited reduced hRAD51 expression, and sixty-two (35{\%}) exhibited p53 overexpression. On the other hand, reduced expression level of BARD1, and of hMSH2 and hMLH1, which are components of DNA mismatch-repair pathway and are involved in colorectal carcinogenesis, was observed respectively in only 10 (6{\%}), 8 (5{\%}) and 3 (2{\%}) cases. The overall frequency of sporadic breast carcinomas with abnormal expression of either BRCA1 or the BRCA1-interactive proteins was 67{\%} (76/113). These results indicate that there may be an important role for the BRCA1-associated DNA-repair pathway, not only in BRCA1-associated breast carcinomas, but also in sporadic breast carcinomas. (C) 2000 Wiley-Liss, Inc.",
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AU - Ogawa, Tomoko

AU - Baer, Richard

AU - Hemmi, Hiromichi

AU - Honda, Kazuo

AU - Yamauchi, Akira

AU - Inamoto, Takashi

AU - Ko, Kohaku

AU - Yazumi, Shujiro

AU - Motoda, Hirotoshi

AU - Kodama, Hiroshi

AU - Noguchi, Shinzaburo

AU - Gazdar, Adi F.

AU - Yamaoka, Yoshio

AU - Takahashi, Rei

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AB - Breast cancer is one of the most common malignancies among women. The molecular mechanisms involved in breast carcinogenesis, however, remain to be elucidated. Although somatic mutation of BRCA1 is rare, BRCA1 protein expression is reduced in about 30% of sporadic breast carcinomas (Yoshikawa et al., Clin. Cancer Res., 5:1249-1261, 1999), indicating its possible involvement even in sporadic breast carcinogenesis. Among the BRCA1-interactive proteins are hRAD51 (a human homologue of Escherichia coli rec A protein), BARD1 (BRCA1-associated RING domain 1) and p53, all of which are involved in DNA repair. We have analyzed the expression patterns of the hRAD51, BARD1 and p53 proteins in five breast cancer cell lines, including a BRCA1-deficient cell line, and in 179 breast cancer tissue samples from Japanese women, including 113 sporadic, 47 hereditary (i.e., BRCA1 status unknown), and 19 BRCA1-associated cases. Of the 179 breast carcinomas, fifty-four (30%) exhibited reduced hRAD51 expression, and sixty-two (35%) exhibited p53 overexpression. On the other hand, reduced expression level of BARD1, and of hMSH2 and hMLH1, which are components of DNA mismatch-repair pathway and are involved in colorectal carcinogenesis, was observed respectively in only 10 (6%), 8 (5%) and 3 (2%) cases. The overall frequency of sporadic breast carcinomas with abnormal expression of either BRCA1 or the BRCA1-interactive proteins was 67% (76/113). These results indicate that there may be an important role for the BRCA1-associated DNA-repair pathway, not only in BRCA1-associated breast carcinomas, but also in sporadic breast carcinomas. (C) 2000 Wiley-Liss, Inc.

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