Accessory cell function of human endothelial cells. I. A subpopulation of Ia positive cells is required for antigen presentation

G. Nunez, E. J. Ball, P. Stastny

Research output: Contribution to journalArticle

72 Citations (Scopus)

Abstract

The expression of class I and class II HLA antigens on preparations of human endothelial cells, isolated from umbilical cord veins, was investigated by immunofluorescence. While virtually all endothelial cells expressed class I antigens, less than 1% were positive for class II antigens, as detected with a panel of 10 different monoclonal antibodies. Antigen specific T cell lines proliferated in response to mumps antigen in the presence of endothelial cells or blood monocytes from HLA-DR matched donors. However, these T cell lines failed to respond in the absence of accessory cells or when accessory cells from HLA-D-region mismatched cord donors were used. The ability of both monocytes and endothelial cells to present antigen was abolished by treatment of the cells with monoclonal antibodies specific for either class I or class II HLA antigens plus complement. Similar treatment with monoclonal antibodies specific for monocytes greatly reduced antigen presentation by monocytes but failed to inhibit presentation by endothelial cells. These results indicate that preparations of endothelial cells contain a subpopulation of Ia positive cells, distinct from monocytes, which are required for antigen presentation.

Original languageEnglish (US)
Pages (from-to)666-673
Number of pages8
JournalJournal of Immunology
Volume131
Issue number2
StatePublished - 1983

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Antigen Presentation
Endothelial Cells
Monocytes
Histocompatibility Antigens Class II
Monoclonal Antibodies
HLA Antigens
Antigens
HLA-D Antigens
T-Lymphocytes
Cell Line
Mumps
Histocompatibility Antigens Class I
Umbilical Veins
Umbilical Cord
HLA-DR Antigens
Fluorescent Antibody Technique
Therapeutics

ASJC Scopus subject areas

  • Immunology

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Accessory cell function of human endothelial cells. I. A subpopulation of Ia positive cells is required for antigen presentation. / Nunez, G.; Ball, E. J.; Stastny, P.

In: Journal of Immunology, Vol. 131, No. 2, 1983, p. 666-673.

Research output: Contribution to journalArticle

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