Activation of antigen-enriched B cells. I. Purification and response to thymus-independent antigens

E. C. Snow, E. S. Vitetta, J. W. Uhr

Research output: Contribution to journalArticle

51 Scopus citations

Abstract

The development of a procedure for the selection of a large number of functional antigen-binding B cells is described. Forty to 70% of the cells in the enriched population bind antigen, and the antigen-binding can be inhibited by pretreatment with either the free hapten (TNP-Lys) or anti-immunoglobulin. The enriched cells express both sIgM and sIgD and respond to mitogenic stimuli by proliferating and developing into antibody-forming cells. In contrast to normal spleen cells, however, the enriched cells also proliferate in response to sub-mitogenic levels of TNP-Brucella abortus and TNP-LPS.

Original languageEnglish (US)
Pages (from-to)607-613
Number of pages7
JournalJournal of Immunology
Volume130
Issue number2
StatePublished - Jan 1 1983

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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