Activation of Arp2/3 complex-dependent actin polymerization by plant proteins distantly related to Scar/WAVE

Mary Frank, Coumaran Egile, Julia Dyachok, Stevan Djakovic, Michelle Nolasco, Rong Li, Laurie G. Smith

Research output: Contribution to journalArticle

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Abstract

The Arp2/3 complex, a highly conserved nucleator of F-actin polymerization, plays a key role in the regulation of actin dynamics eukaryotic cells. In animal cells and yeasts, Wiskott-Aldrich Syndrome protein (WASP)/suppressor of cAMP receptor (Scar)/WASP family verprolin homologous (WAVE) family proteins activate the Arp2/3 complex in response to localized cues. Like other eukaryotes, plants have an Arp2/3 complex, which has recently been shown to play an important role in F-actin organization and cell morphogenesis. However, no activators of the Arp2/3 complex have been identified in plants, which lack obvious homologs of WASP/Scar/WAVE family proteins. Here, we identify a family of Scar/WAVE-related plant Arp2/3 activators. Like Scar/WAVE proteins, four proteins identified in Arabidopsis thaliana (AtSCAR1 to AtSCAR4) and one in maize (ZmSCAR1) have a C-terminal WASP homology 2 (WH2)/acidic (WA)-verprolin homology/cofilin homology/acidic (VCA)-like domain, which we show can activate the bovine Arp2/3 complex. At their N termini, AtSCAR1 to ATSCAR4, along with a fifth protein lacking a VCA/WA-like domain at its C terminus (At4g18600), are related to the N-terminal Scar homology domains of Scar/WAVE family proteins. Analysis of gene expression patterns suggests functional redundancy among members of the AtSCAR family. Full-length AtSCAR1 and ATSCAR3 proteins and their Scar homology domains bind in vitro to AtBRICK 1 (AtBRKI), the Arabidopsis homolog of HSPC300, a WAVE-binding protein recently identified as a component of a complex implicated in the regulation of Scar/WAVE activity. Thus, AtSCAR proteins are likely to function in association with AtBRK1, and perhaps other Arabidopsis homologs of WAVE complex components, to regulate activation of the Arp2,3 complex in vivo.

Original languageEnglish (US)
Pages (from-to)16379-16384
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume101
Issue number46
DOIs
StatePublished - Nov 16 2004

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Actin-Related Protein 2-3 Complex
Cyclic AMP Receptors
Wiskott-Aldrich Syndrome Protein Family
Plant Proteins
Polymerization
Actins
Wiskott-Aldrich Syndrome Protein
Arabidopsis
Proteins
Cyclic AMP Receptor Protein
Actin Depolymerizing Factors
Gene Expression Profiling
Eukaryotic Cells
Eukaryota
Morphogenesis
Zea mays
Cues
Carrier Proteins
Yeasts

Keywords

  • BRICK1
  • HSPC300
  • WAVE complex

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Activation of Arp2/3 complex-dependent actin polymerization by plant proteins distantly related to Scar/WAVE. / Frank, Mary; Egile, Coumaran; Dyachok, Julia; Djakovic, Stevan; Nolasco, Michelle; Li, Rong; Smith, Laurie G.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, No. 46, 16.11.2004, p. 16379-16384.

Research output: Contribution to journalArticle

Frank, Mary ; Egile, Coumaran ; Dyachok, Julia ; Djakovic, Stevan ; Nolasco, Michelle ; Li, Rong ; Smith, Laurie G. / Activation of Arp2/3 complex-dependent actin polymerization by plant proteins distantly related to Scar/WAVE. In: Proceedings of the National Academy of Sciences of the United States of America. 2004 ; Vol. 101, No. 46. pp. 16379-16384.
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