TY - JOUR
T1 - Activation of the nuclear receptor peroxisome proliferator-activated receptor γ promotes brown adipocyte differentiation
AU - Tai, Tzu Ann C
AU - Jennermann, Caroline
AU - Brown, Kathleen K.
AU - Oliver, Beverly B.
AU - MacGinnitie, Marissa A.
AU - Wilkison, William O.
AU - Roger Brown, H.
AU - Lehmann, Jürgen M.
AU - Kliewer, Steven A.
AU - Morris, David C.
AU - Graves, Reed A.
PY - 1996
Y1 - 1996
N2 - Brown adipose tissue (BAT) functions in non-shivering and diet-induced thermogenesis via its capacity for uncoupled mitochondrial respiration. BAT dysfunction in rodents is associated with severe defects in energy homeostasis, resulting in obesity and hyperglycemia. Here, we report that the nuclear receptor peroxisome proliferator-activated receptor γ (PPARγ), a prostaglandin-activated transcription factor recently implicated as a central regulator of white adipose tissue differentiation, also regulates brown adipocyte function. PPARγ is abundantly expressed in both embryonic and adult BAT. Treatment of CD-1 rats with the PPARγ-selective ligand BRL49653, an anti-diabetic drug of the thiazolidinedione class, results in marked increases in the mass of interscapular BAT. In vitro, BRL49653 induces the terminal differentiation of the brown preadipocyte cell line HIB-1B as judged by both changes in cell morphology and expression of uncoupling protein and other adipocyte-specific mRNAs. These data demonstrate that PPARγ is a key regulatory factor in brown adipocytes and suggest that PPARγ functions not only in the storage of excess energy in white adipose tissue but also in its dissipation in BAT.
AB - Brown adipose tissue (BAT) functions in non-shivering and diet-induced thermogenesis via its capacity for uncoupled mitochondrial respiration. BAT dysfunction in rodents is associated with severe defects in energy homeostasis, resulting in obesity and hyperglycemia. Here, we report that the nuclear receptor peroxisome proliferator-activated receptor γ (PPARγ), a prostaglandin-activated transcription factor recently implicated as a central regulator of white adipose tissue differentiation, also regulates brown adipocyte function. PPARγ is abundantly expressed in both embryonic and adult BAT. Treatment of CD-1 rats with the PPARγ-selective ligand BRL49653, an anti-diabetic drug of the thiazolidinedione class, results in marked increases in the mass of interscapular BAT. In vitro, BRL49653 induces the terminal differentiation of the brown preadipocyte cell line HIB-1B as judged by both changes in cell morphology and expression of uncoupling protein and other adipocyte-specific mRNAs. These data demonstrate that PPARγ is a key regulatory factor in brown adipocytes and suggest that PPARγ functions not only in the storage of excess energy in white adipose tissue but also in its dissipation in BAT.
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U2 - 10.1074/jbc.271.47.29909
DO - 10.1074/jbc.271.47.29909
M3 - Article
C2 - 8939934
AN - SCOPUS:10544229796
SN - 0021-9258
VL - 271
SP - 29909
EP - 29914
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
IS - 47
ER -