ADCY7 supports development of acute myeloid leukemia

Chunling Li, Jingjing Xie, Zhigang Lu, Chen Chen, Yancun Yin, Renhui Zhan, Yi Fang, Xuemei Hu, Chengcheng Zhang

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Acute myeloid leukemia (AML) is the most common adult acute leukemia. Despite treatment, the majority of the AML patients relapse within 5 years. In silico analysis of several available databases of AML patients showed that the expression of adenylate cyclase 7 (ADCY7) significantly inversely correlates with the overall survival of AML patients. To determine whether ADCY7 supports AML development, we employed an shRNA-encoding lentivirus system to inhibit adcy7 expression in human AML cells including U937, MV4-11, and THP-1 cells. The ADCY7 deficiency resulted in decreased cell growth, elevated apoptosis, and lower c-Myc expression of these leukemia cells. This indicates that G protein-coupled receptor signaling contributes to AML pathogenesis. Our study suggests that inhibition of ADCY7 may be novel strategy for treating leukemia.

Original languageEnglish (US)
Article number34335
Pages (from-to)47-52
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume465
Issue number1
DOIs
StatePublished - Jul 26 2015

Fingerprint

Adenylyl Cyclases
Acute Myeloid Leukemia
Leukemia
Cell growth
G-Protein-Coupled Receptors
Small Interfering RNA
Lentivirus
Apoptosis
Myeloid Cells
Computer Simulation
Databases
Recurrence
Survival
Growth

Keywords

  • Acute myeloid leukemia
  • ADCY7
  • Apoptosis
  • Cell growth
  • G protein-coupled receptor
  • shRNA

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Molecular Biology

Cite this

ADCY7 supports development of acute myeloid leukemia. / Li, Chunling; Xie, Jingjing; Lu, Zhigang; Chen, Chen; Yin, Yancun; Zhan, Renhui; Fang, Yi; Hu, Xuemei; Zhang, Chengcheng.

In: Biochemical and Biophysical Research Communications, Vol. 465, No. 1, 34335, 26.07.2015, p. 47-52.

Research output: Contribution to journalArticle

Li, C, Xie, J, Lu, Z, Chen, C, Yin, Y, Zhan, R, Fang, Y, Hu, X & Zhang, C 2015, 'ADCY7 supports development of acute myeloid leukemia', Biochemical and Biophysical Research Communications, vol. 465, no. 1, 34335, pp. 47-52. https://doi.org/10.1016/j.bbrc.2015.07.123
Li, Chunling ; Xie, Jingjing ; Lu, Zhigang ; Chen, Chen ; Yin, Yancun ; Zhan, Renhui ; Fang, Yi ; Hu, Xuemei ; Zhang, Chengcheng. / ADCY7 supports development of acute myeloid leukemia. In: Biochemical and Biophysical Research Communications. 2015 ; Vol. 465, No. 1. pp. 47-52.
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