Adenosine induces inositol 1,4,5-trisphosphate receptor-mediated mobilization of intracellular calcium stores in basal forebrain cholinergic neurons

Radhika Basheer, Elda Arrigoni, Hemant S. Thatte, Robert W. Greene, Indu S. Ambudkar, Robert W. McCarley

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

In the cholinergic basal forebrain, we found previously that the extracellular adenosine concentration increase that accompanies sleep deprivation, acting via the A1 receptor, led to activation of the transcription factor nuclear factor-κB and to the upregulation of A1 adenosine receptor mRNA. We thus began to examine intracellular signaling mechanisms. We report here that adenosine, acting in a dose-dependent manner and predominantly via A1 receptors, stimulated IP3 receptor-regulated calcium release from intracellular stores. To the best of our knowledge, this calcium signaling pathway effect is a novel action of the Gi-coupled A1 adenosine receptor in neurons. Moreover, this calcium mobilization was not seen at all in noncholinergic neurons but was present in a large proportion of cholinergic neurons. These data suggest a potential role for a calcium-signaling pathway in adenosine-induced long-term effects of sleep deprivation and a key role for cholinergic neurons in this process.

Original languageEnglish (US)
Pages (from-to)7680-7686
Number of pages7
JournalJournal of Neuroscience
Volume22
Issue number17
StatePublished - Sep 1 2002

Fingerprint

Inositol 1,4,5-Trisphosphate Receptors
Cholinergic Neurons
Adenosine
Adenosine A1 Receptors
Sleep Deprivation
Calcium Signaling
Calcium
Neurons
Cholinergic Agents
Transcription Factors
Up-Regulation
Messenger RNA
Basal Forebrain

Keywords

  • A adenosine receptor
  • Adenosine
  • Cholinergic basal forebrain
  • Inositol 1,4,5-trisphosphate receptor activation
  • Intracellular calcium mobilization
  • Sleep deprivation

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Adenosine induces inositol 1,4,5-trisphosphate receptor-mediated mobilization of intracellular calcium stores in basal forebrain cholinergic neurons. / Basheer, Radhika; Arrigoni, Elda; Thatte, Hemant S.; Greene, Robert W.; Ambudkar, Indu S.; McCarley, Robert W.

In: Journal of Neuroscience, Vol. 22, No. 17, 01.09.2002, p. 7680-7686.

Research output: Contribution to journalArticle

Basheer, Radhika ; Arrigoni, Elda ; Thatte, Hemant S. ; Greene, Robert W. ; Ambudkar, Indu S. ; McCarley, Robert W. / Adenosine induces inositol 1,4,5-trisphosphate receptor-mediated mobilization of intracellular calcium stores in basal forebrain cholinergic neurons. In: Journal of Neuroscience. 2002 ; Vol. 22, No. 17. pp. 7680-7686.
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