Aging alters circadian and light-induced expression of clock genes in golden hamsters

Daniel E. Kolker, Hiroo Fukuyama, David S. Huang, Joseph S. Takahashi, Teresa H. Horton, Fred W. Turek

Research output: Contribution to journalArticle

120 Citations (Scopus)

Abstract

Aging alters numerous aspects of circadian biology, including the amplitude of rhythms generated by the suprachiasmatic nuclei (SCN) of the hypothalamus, the site of the central circadian pacemaker in mammals, and the response of the pacemaker to environmental stimuli such as light. Although previous studies have described molecular correlates of these behavioral changes, to date only 1 study in rats has attempted to determine if there are age-related changes in the expression of genes that comprise the circadian clock itself. We used in situ hybridization to examine the effects of age on the circadian pattern of expression of a subset of the genes that comprise the molecular machinery of the circadian clock in golden hamsters. Here we report that age alters the 24-h expression profile of Clock and its binding partner Bmal1 in the hamster SCN. There is no effect of age on the 24-h profile of either Per1 or Per2 when hamsters are housed in constant darkness. We also found that light pulses, which induce smaller phase shifts in old animals than in young, lead to decreased induction of Per1, but not of Per2, in the SCN of old hamsters.

Original languageEnglish (US)
Pages (from-to)159-169
Number of pages11
JournalJournal of Biological Rhythms
Volume18
Issue number2
DOIs
StatePublished - Apr 1 2003

Fingerprint

Suprachiasmatic Nucleus
Mesocricetus
Cricetinae
Circadian Clocks
hamsters
Gene Expression
Light
circadian rhythm
genes
Darkness
gene expression
Hypothalamus
In Situ Hybridization
Mammals
hypothalamus
in situ hybridization
mammals
Biological Sciences
Genes
Mesocricetus auratus

Keywords

  • Aging
  • Bmal1
  • Circadian rhythm
  • Clock
  • Gene expression
  • Hamster
  • Light induced
  • Per1
  • Per2

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Physiology
  • Physiology (medical)

Cite this

Aging alters circadian and light-induced expression of clock genes in golden hamsters. / Kolker, Daniel E.; Fukuyama, Hiroo; Huang, David S.; Takahashi, Joseph S.; Horton, Teresa H.; Turek, Fred W.

In: Journal of Biological Rhythms, Vol. 18, No. 2, 01.04.2003, p. 159-169.

Research output: Contribution to journalArticle

Kolker, Daniel E. ; Fukuyama, Hiroo ; Huang, David S. ; Takahashi, Joseph S. ; Horton, Teresa H. ; Turek, Fred W. / Aging alters circadian and light-induced expression of clock genes in golden hamsters. In: Journal of Biological Rhythms. 2003 ; Vol. 18, No. 2. pp. 159-169.
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