Allopeptide-pulsed dendritic cells and composite tissue allograft survival

Vu T. Nguyen, Aurele Taieb, Justin M. Sacks, Jignesh V. Unadkat, Julio A. Clavijo, Haena Kim, Maryam Feili-Hariri, W. P.Andrew Lee

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

Composite tissue allografts (CTAs) contain their own reservoir of vascularized bone marrow, offering novel aspects for the induction of donor-specific tolerance. Additionally, the manipulation of recipient dendritic cells, pulsed with donor allopeptide, has been shown to engender solid organ allograft survival. To exploit these modalities, we have developed a protocol utilizing injection of recipient bone marrow-derived dendritic cells (BMDCs) pulsed with a donor-derived peptide for use in CTA transplantation. Six days prior to orthotopic hind-limb transplantation, Lewis rats received IV injection of donor allopeptide-pulsed, recipient BMDCs, in conjunction with a single dose of anti-lymphocyte serum. Control groups displayed signs of allograft rejection within 5 days postoperatively. Animals within the primary experimental cohort demonstrated prolongation of graft survival to an average of 8 days, and exhibited low numbers of donor T cells. The use of BMDCs in conjunction with transient immunosuppression has potential therapeutic application for induction of donor-antigen-specific tolerance to hind limb allografts.

Original languageEnglish (US)
Pages (from-to)105-111
Number of pages7
JournalMicrosurgery
Volume27
Issue number2
DOIs
StatePublished - 2007
Externally publishedYes

ASJC Scopus subject areas

  • Surgery

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