Allopurinol preserves cerebral energy metabolism during perinatal hypoxia-ischemia: A 31P NMR study in unanesthetized immature rats

Gerald D. Williams; Charles Palmer; Daniel F. Heitjan; Michael B. Smith

doi:10.1016/0304-3940(92)90726-N

Allopurinol preserves cerebral energy metabolism during perinatal hypoxia-ischemia: A ³¹P NMR study in unanesthetized immature rats

Gerald D. Williams, Charles Palmer, Daniel F. Heitjan, Michael B. Smith

Research output: Contribution to journal › Article › peer-review

51 Scopus citations

Abstract

The effects of high dose allopurinol (ALLOP) pretreatment on the cerebral energy metabolism of unanesthetized 7-day-postnatal rats during exposure to 3 h of cerebral hypoxia-ischemia were serially quantitated using non-invasive ³¹P NMR spectroscopy. Adenosine triphosphate, integrated over the last 2 h of hypoxia and expressed as a fraction of baseline, was 0.73±0.16 with ALLOP pretreatment (200 mg/kg s.c.) compared to 0.52±0.05 for saline pretreatment (P=0.001). Inorganic phosphate/phosphocreatine (P_i/PCr), integrated over the same time interval, was 2.63±1.23 relative to baseline with ALLOP versus 5.13±1.45 for saline-treated pups (P<0.0005). We suggest that the neuroprotection achieved with high dose ALLOP pretreatment may be attributed in part to preservation of energy metabolites.

Original language	English (US)
Pages (from-to)	103-106
Number of pages	4
Journal	Neuroscience Letters
Volume	144
Issue number	1-2
DOIs	https://doi.org/10.1016/0304-3940(92)90726-N
State	Published - Sep 14 1992

Keywords

Adenosine triphosphate
Allopurinol
Hypoxia-ischemia
Immature animal
Neuroprotective
Nuclear magnetic resonance
Rat brain
Xanthine oxidase

ASJC Scopus subject areas

General Neuroscience

Access to Document

10.1016/0304-3940(92)90726-N

Cite this

@article{9a5330eb3bee41e1b627f6fcd3aeb11e,

title = "Allopurinol preserves cerebral energy metabolism during perinatal hypoxia-ischemia: A 31P NMR study in unanesthetized immature rats",

abstract = "The effects of high dose allopurinol (ALLOP) pretreatment on the cerebral energy metabolism of unanesthetized 7-day-postnatal rats during exposure to 3 h of cerebral hypoxia-ischemia were serially quantitated using non-invasive 31P NMR spectroscopy. Adenosine triphosphate, integrated over the last 2 h of hypoxia and expressed as a fraction of baseline, was 0.73±0.16 with ALLOP pretreatment (200 mg/kg s.c.) compared to 0.52±0.05 for saline pretreatment (P=0.001). Inorganic phosphate/phosphocreatine (Pi/PCr), integrated over the same time interval, was 2.63±1.23 relative to baseline with ALLOP versus 5.13±1.45 for saline-treated pups (P<0.0005). We suggest that the neuroprotection achieved with high dose ALLOP pretreatment may be attributed in part to preservation of energy metabolites.",

keywords = "Adenosine triphosphate, Allopurinol, Hypoxia-ischemia, Immature animal, Neuroprotective, Nuclear magnetic resonance, Rat brain, Xanthine oxidase",

author = "Williams, {Gerald D.} and Charles Palmer and Heitjan, {Daniel F.} and Smith, {Michael B.}",

year = "1992",

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AU - Williams, Gerald D.

AU - Palmer, Charles

AU - Heitjan, Daniel F.

AU - Smith, Michael B.

PY - 1992/9/14

Y1 - 1992/9/14

N2 - The effects of high dose allopurinol (ALLOP) pretreatment on the cerebral energy metabolism of unanesthetized 7-day-postnatal rats during exposure to 3 h of cerebral hypoxia-ischemia were serially quantitated using non-invasive 31P NMR spectroscopy. Adenosine triphosphate, integrated over the last 2 h of hypoxia and expressed as a fraction of baseline, was 0.73±0.16 with ALLOP pretreatment (200 mg/kg s.c.) compared to 0.52±0.05 for saline pretreatment (P=0.001). Inorganic phosphate/phosphocreatine (Pi/PCr), integrated over the same time interval, was 2.63±1.23 relative to baseline with ALLOP versus 5.13±1.45 for saline-treated pups (P<0.0005). We suggest that the neuroprotection achieved with high dose ALLOP pretreatment may be attributed in part to preservation of energy metabolites.

AB - The effects of high dose allopurinol (ALLOP) pretreatment on the cerebral energy metabolism of unanesthetized 7-day-postnatal rats during exposure to 3 h of cerebral hypoxia-ischemia were serially quantitated using non-invasive 31P NMR spectroscopy. Adenosine triphosphate, integrated over the last 2 h of hypoxia and expressed as a fraction of baseline, was 0.73±0.16 with ALLOP pretreatment (200 mg/kg s.c.) compared to 0.52±0.05 for saline pretreatment (P=0.001). Inorganic phosphate/phosphocreatine (Pi/PCr), integrated over the same time interval, was 2.63±1.23 relative to baseline with ALLOP versus 5.13±1.45 for saline-treated pups (P<0.0005). We suggest that the neuroprotection achieved with high dose ALLOP pretreatment may be attributed in part to preservation of energy metabolites.

KW - Adenosine triphosphate

KW - Allopurinol

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KW - Immature animal

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KW - Rat brain

KW - Xanthine oxidase

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