Alterations in hepatic glucose and energy metabolism as a result of calorie and Carbohydrate restriction

Jeffrey D. Browning, Brian Weis, Jeannie Davis, Santhosh Satapati, Matthew Merritt, Craig R. Malloy, Shawn C. Burgess

Research output: Contribution to journalArticle

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Abstract

Carbohydrate restriction is a common weight-loss approach that modifies hepatic metabolism by increasing gluconeogenesis (GNG) and ketosis. Because little is known about the effect of carbohydrate restriction on the origin of gluconeogenic precursors (GNG from glycerol [GNGglycerol] and GNG from lactate/amino acids [GNGphosphoenolpyruvate {PEP}]) or its consequence to hepatic energy homeostasis, we studied these parameters in a group of overweight/obese subjects undergoing weight-loss via dietary restriction. We used 2H and 13C tracers and nuclear magnetic resonance spectroscopy to measure the sources of hepatic glucose and tricarboxylic acid (TCA) cycle flux in weight-stable subjects (n=7) and subjects following carbohydrate restriction (n = 7) or calorie restriction (n = 7). The majority of hepatic glucose production in carbohydrate restricted subjects came from GNGPEP. The contribution of glycerol to GNG was similar in all groups despite evidence of increased fat oxidation in carbohydrate restricted subjects. A strong correlation between TCA cycle flux and GNGPEP was found, though the reliance on TCA cycle energy production for GNG was attenuated in subjects undergoing carbohydrate restriction. Together, these data imply that the TCA cycle is the energetic patron of GNG. However, the relationship between these two pathways is modified by carbohydrate restriction, suggesting an increased reliance of the hepatocyte on energy generated outside of the TCA cycle when GNGPEP is maximal. Conclusion: Carbohydrate restriction modifies hepatic GNG by increasing reliance on substrates like lactate or amino acids but not glycerol. This modification is associated with a reorganization of hepatic energy metabolism suggestive of enhanced hepatic β-oxidation.

Original languageEnglish (US)
Pages (from-to)1487-1496
Number of pages10
JournalHepatology
Volume48
Issue number5
DOIs
StatePublished - Nov 2008

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Gluconeogenesis
Energy Metabolism
Citric Acid Cycle
Carbohydrates
Glucose
Liver
Weight Loss
Lactic Acid
Amino Acids
Ketosis
Hepatocytes
Homeostasis
Magnetic Resonance Spectroscopy
Fats
Weights and Measures

ASJC Scopus subject areas

  • Hepatology
  • Medicine(all)

Cite this

Alterations in hepatic glucose and energy metabolism as a result of calorie and Carbohydrate restriction. / Browning, Jeffrey D.; Weis, Brian; Davis, Jeannie; Satapati, Santhosh; Merritt, Matthew; Malloy, Craig R.; Burgess, Shawn C.

In: Hepatology, Vol. 48, No. 5, 11.2008, p. 1487-1496.

Research output: Contribution to journalArticle

Browning, Jeffrey D. ; Weis, Brian ; Davis, Jeannie ; Satapati, Santhosh ; Merritt, Matthew ; Malloy, Craig R. ; Burgess, Shawn C. / Alterations in hepatic glucose and energy metabolism as a result of calorie and Carbohydrate restriction. In: Hepatology. 2008 ; Vol. 48, No. 5. pp. 1487-1496.
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