Alterations in transcription factor binding in radioresistant human melanoma cells after ionizing radiation

W. M. Sahijdak, C. R. Yang, J. S. Zuckerman, M. Meyers, D. A. Boothman

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

We analyzed alterations in transcription factor binding to specific, known promoter DNA consensus sequences between irradiated and unirradiated radioresistant human melanoma (U1-Mel) cells. The goal of this study was to begin to investigate which transcription factors and DNA-binding sites are responsible for the induction of specific transcripts and proteins after ionizing radiation (Boothman et al., Proc. Natl. Acad. Sci. USA 90, 7200, 1993). Transcription factor binding was observed using DNA band-shift assays and oligonucleotide competition analyses. Confluence-arrested U1-Mel cells were irradiated (4.5 Gy) and harvested at 4 h. Double-stranded oligonucleotides containing known DNA-binding consensus sites for specific transcription factors were used. Increased DNA-binding activity after ionizing radiation was noted with oligonucleotides containing the CREB, NF- κB and Sp1 consensus sites. Increased DNA binding activity after ionizing radiation was noted with oligonucleotides containing the CREB, NF-κB and Sp1 consensus sites. No changes in protein binding to AP-1, AP-2, AP-3, or CTF/NF1, GRE or Oct-1 consensus sequences were noted. X-ray activation of select transcription factors, which bind certain consensus sites in promoters, may cause specific induction or repression of gene transcription.

Original languageEnglish (US)
JournalRadiation Research
Volume138
Issue number1 SUPPL.
StatePublished - 1994

Fingerprint

melanoma
Ionizing Radiation
ionizing radiation
Melanoma
Transcription Factors
transcription factors
deoxyribonucleic acid
oligonucleotides
Oligonucleotides
DNA
cells
Consensus Sequence
consensus sequence
Binding Sites
induction
promoter regions
proteins
Transcription Factor AP-1
Protein Binding
protein binding

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biophysics
  • Radiology Nuclear Medicine and imaging
  • Radiation

Cite this

Sahijdak, W. M., Yang, C. R., Zuckerman, J. S., Meyers, M., & Boothman, D. A. (1994). Alterations in transcription factor binding in radioresistant human melanoma cells after ionizing radiation. Radiation Research, 138(1 SUPPL.).

Alterations in transcription factor binding in radioresistant human melanoma cells after ionizing radiation. / Sahijdak, W. M.; Yang, C. R.; Zuckerman, J. S.; Meyers, M.; Boothman, D. A.

In: Radiation Research, Vol. 138, No. 1 SUPPL., 1994.

Research output: Contribution to journalArticle

Sahijdak, WM, Yang, CR, Zuckerman, JS, Meyers, M & Boothman, DA 1994, 'Alterations in transcription factor binding in radioresistant human melanoma cells after ionizing radiation', Radiation Research, vol. 138, no. 1 SUPPL..
Sahijdak, W. M. ; Yang, C. R. ; Zuckerman, J. S. ; Meyers, M. ; Boothman, D. A. / Alterations in transcription factor binding in radioresistant human melanoma cells after ionizing radiation. In: Radiation Research. 1994 ; Vol. 138, No. 1 SUPPL.
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