Altered regulation of brain-derived neurotrophic factor protein in hippocampus following slice preparation

S. C. Danzer, E. Pan, S. Nef, L. F. Parada, J. O. McNamara

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Brain-derived neurotrophic factor (BDNF) and its cognate receptor tyrosine kinase B (TrkB) play important roles in regulating survival, structure, and function of CNS neurons. One method of studying the functions of these molecules has utilized in vitro hippocampal slice preparations. An important caveat to using slices, however, is that slice preparation itself might alter the expression of BDNF, thereby confounding experimental results. To address this concern, BDNF immunoreactivity was examined in rodent slices using two different methods of slice preparation. Rapid and anatomically selective regulation of BDNF content followed slice preparation using both methodologies; however, different patterns of altered BDNF immunoreactivity were observed. First, in cultured slices, BDNF content decreased in the dentate molecular layer and increased in the CA3 pyramidal cell layer and the mossy fiber pathway of the hippocampus after 30 min. Furthermore, an initially "punctate" pattern of BDNF labeling observed in the mossy fiber pathway of control sections changed to homogenous labeling of the pathway in vitro. In contrast to these findings, slices prepared as for acute slice physiology exhibited no change in BDNF content in the molecular layer and mossy fiber pathway 30 min after slicing, but exhibited significant increases in the dentate granule and CA3 pyramidal cell layers. These findings demonstrate that BDNF protein content is altered following slice preparation, that different methods of slice preparation produce different patterns of BDNF regulation, and raise the possibility that BDNF release and TrkB activation may also be regulated. These consequences of hippocampal slice preparation may confound analyses of exogenous or endogenous BDNF on hippocampal neuronal structure or function.

Original languageEnglish (US)
Pages (from-to)859-869
Number of pages11
JournalNeuroscience
Volume126
Issue number4
DOIs
StatePublished - 2004

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Brain-Derived Neurotrophic Factor
Nerve Growth Factors
Hippocampus
Hippocampal CA3 Region
Receptor Protein-Tyrosine Kinases
Protein-Tyrosine Kinases
Rodentia

Keywords

  • 0 min in ACSF holding chamber
  • ACSF
  • artificial cerebrospinal fluid
  • BDNF
  • BDNF knockout mice
  • brain-derived neurotrophic factor
  • dentate granule cell
  • GDNF
  • glial-cell line-derived neurotrophic factor
  • HCT=0
  • hippocampal slice
  • Stoppini method
  • trauma
  • TrkB

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Altered regulation of brain-derived neurotrophic factor protein in hippocampus following slice preparation. / Danzer, S. C.; Pan, E.; Nef, S.; Parada, L. F.; McNamara, J. O.

In: Neuroscience, Vol. 126, No. 4, 2004, p. 859-869.

Research output: Contribution to journalArticle

Danzer, S. C. ; Pan, E. ; Nef, S. ; Parada, L. F. ; McNamara, J. O. / Altered regulation of brain-derived neurotrophic factor protein in hippocampus following slice preparation. In: Neuroscience. 2004 ; Vol. 126, No. 4. pp. 859-869.
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