An anti-CD45RO immunotoxin eliminates T cells latently infected with HIV-1 in vitro

Cynthia McCoig, Gregory Van Dyke, Chin Sheng Chou, Louis J. Picker, Octavio Ramilo, Ellen S. Vitetta

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Abstract

Despite the success of highly active antiretroviral therapy (HAART) in lowering circulating HIV-1 to undetectable levels in most infected individuals, several studies have documented the presence of a small reservoir of latently infected cells in HAART patients, the majority of which are CD45RO+ memory T cells. We previously have demonstrated that latently infected, replication-competent cells can be generated in vitro after eliminating CD25+ cells with an immunotoxin (IT). The present study was designed to determine whether these latent cells could be eliminated by an anti-CD45RO IT. Our results indicate that the anti-CD45RO IT eliminates >99%, of either M-tropic or T-tropic virus produced by the latently infected cells after mitogen stimulation. This IT also appears to be as effective as the anti-CD25 IT in eliminating the activated, HIV-1-producing cells. In contrast, the anti-CD45RO IT does not kill CD45RA+ naive cells. Further studies using cells from HIV-1-infected individuals on HAART will be necessary to determine the potential clinical utility of this IT.

Original languageEnglish (US)
Pages (from-to)11482-11485
Number of pages4
JournalProceedings of the National Academy of Sciences of the United States of America
Volume96
Issue number20
DOIs
StatePublished - Sep 28 1999

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Immunotoxins
HIV-1
T-Lymphocytes
Highly Active Antiretroviral Therapy
In Vitro Techniques
Mitogens
Viruses

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

An anti-CD45RO immunotoxin eliminates T cells latently infected with HIV-1 in vitro. / McCoig, Cynthia; Van Dyke, Gregory; Chou, Chin Sheng; Picker, Louis J.; Ramilo, Octavio; Vitetta, Ellen S.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 96, No. 20, 28.09.1999, p. 11482-11485.

Research output: Contribution to journalArticle

McCoig, Cynthia ; Van Dyke, Gregory ; Chou, Chin Sheng ; Picker, Louis J. ; Ramilo, Octavio ; Vitetta, Ellen S. / An anti-CD45RO immunotoxin eliminates T cells latently infected with HIV-1 in vitro. In: Proceedings of the National Academy of Sciences of the United States of America. 1999 ; Vol. 96, No. 20. pp. 11482-11485.
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