An atypical pattern of accumulation of scopolamine and other tropane alkaloids and expression of alkaloid pathway genes in Hyoscyamus senecionis

Esmail Dehghan, Farajollah Shahriari Ahmadi, Elnaz Ghotbi Ravandi, Darwin W. Reed, Patrick S. Covello, Ahmad Reza Bahrami

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

A cDNA encoding hyoscyamine 6β-hydroxylase (H6H, EC 1.14.11.11), a bifunctional enzyme catalyzing the last two steps in the scopolamine biosynthetic pathway, was isolated from Hyoscyamus senecionis, a medicinal plant endemic to the Iranian plateau. Expression analysis indicates that Hsh6h is expressed in all tested organs of H. senecionis including roots, rhizomes, leaves, stems and flowers unlike the other tropane alkaloid producing species. In parallel to this, in leaves, levels of scopolamine, the product of H6H, were higher than the substrate hyoscyamine. These data suggest that not only does the conversion of hyoscyamine to scopolamine take place in the root, followed by translocation to aerial parts, but also accumulated hyoscyamine in the aerial parts may be converted to scopolamine by activity of HsH6H. Analysis of expression profiles of putrescine N-methyltransferase and tropinone reductase I and II genes also indicates the organ-independent expression of these genes. Here we also introduce H.senecionis as an important tropane alkaloid producing species with its thick underground parts as a source of hyoscyamine, while its leaves can be considered as a source of scopolamine.

Original languageEnglish (US)
Pages (from-to)188-194
Number of pages7
JournalPlant Physiology and Biochemistry
Volume70
DOIs
StatePublished - Sep 2013
Externally publishedYes

Keywords

  • BSTFA
  • Expression profile
  • GC
  • GC-MS
  • Hyoscyamine 6β-hydroxylase (H6H)
  • Hyoscyamus senecionis
  • IPTG
  • MS
  • ORF
  • PMT
  • Solanaceae
  • TMCS
  • TRI
  • TRII
  • Tropane alkaloids
  • X-Gal

ASJC Scopus subject areas

  • Physiology
  • Genetics
  • Plant Science

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