An improved monomeric infrared fluorescent protein for neuronal and tumour brain imaging

Dan Yu, William Clay Gustafson, Chun Han, Céline Lafaye, Marjolaine Noirclerc-Savoye, Woo Ping Ge, Desiree A. Thayer, Hai Huang, Thomas B. Kornberg, Antoine Royant, Lily Yeh Jan, Yuh Nung Jan, William A. Weiss, Xiaokun Shu

Research output: Contribution to journalArticle

75 Citations (Scopus)

Abstract

Infrared fluorescent proteins (IFPs) are ideal for in vivo imaging, and monomeric versions of these proteins can be advantageous as protein tags or for sensor development. In contrast to GFP, which requires only molecular oxygen for chromophore maturation, phytochrome-derived IFPs incorporate biliverdin (BV) as the chromophore. However, BV varies in concentration in different cells and organisms. Here we engineered cells to express the haeme oxygenase responsible for BV biosynthesis and a brighter monomeric IFP mutant (IFP2.0). Together, these tools improve the imaging capabilities of IFP2.0 compared with monomeric IFP1.4 and dimeric iRFP. By targeting IFP2.0 to the plasma membrane, we demonstrate robust labelling of neuronal processes in Drosophila larvae. We also show that this strategy improves the sensitivity when imaging brain tumours in whole mice. Our work shows promise in the application of IFPs for protein labelling and in vivo imaging.

Original languageEnglish (US)
Pages (from-to)3626
Number of pages1
JournalNature Communications
Volume5
DOIs
StatePublished - 2014

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Neuroimaging
brain
Tumors
Brain
tumors
Biliverdine
Infrared radiation
proteins
Imaging techniques
Neoplasms
Proteins
Chromophores
Labeling
chromophores
marking
Phytochrome
phytochrome
Heme Oxygenase (Decyclizing)
Molecular oxygen
larvae

ASJC Scopus subject areas

  • Medicine(all)

Cite this

An improved monomeric infrared fluorescent protein for neuronal and tumour brain imaging. / Yu, Dan; Gustafson, William Clay; Han, Chun; Lafaye, Céline; Noirclerc-Savoye, Marjolaine; Ge, Woo Ping; Thayer, Desiree A.; Huang, Hai; Kornberg, Thomas B.; Royant, Antoine; Jan, Lily Yeh; Jan, Yuh Nung; Weiss, William A.; Shu, Xiaokun.

In: Nature Communications, Vol. 5, 2014, p. 3626.

Research output: Contribution to journalArticle

Yu, D, Gustafson, WC, Han, C, Lafaye, C, Noirclerc-Savoye, M, Ge, WP, Thayer, DA, Huang, H, Kornberg, TB, Royant, A, Jan, LY, Jan, YN, Weiss, WA & Shu, X 2014, 'An improved monomeric infrared fluorescent protein for neuronal and tumour brain imaging', Nature Communications, vol. 5, pp. 3626. https://doi.org/10.1038/ncomms4626
Yu, Dan ; Gustafson, William Clay ; Han, Chun ; Lafaye, Céline ; Noirclerc-Savoye, Marjolaine ; Ge, Woo Ping ; Thayer, Desiree A. ; Huang, Hai ; Kornberg, Thomas B. ; Royant, Antoine ; Jan, Lily Yeh ; Jan, Yuh Nung ; Weiss, William A. ; Shu, Xiaokun. / An improved monomeric infrared fluorescent protein for neuronal and tumour brain imaging. In: Nature Communications. 2014 ; Vol. 5. pp. 3626.
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