Analysis of cell viability and differential activity of mouse hepatocytes under 3D and 2D culture in agarose gel

Hirohiko Ise, Seiji Takashima, Masato Nagaoka, Anwarul Ferdous, Toshihiro Akaike

Research output: Contribution to journalArticle

15 Scopus citations

Abstract

Three-dimensional (3D) and two-dimensional (2D) cultures of hepatocytes in various concentrations (0.3-0.7%) of agarose gel revealed that the hepatocytes under 3D cultures in 0.3% agarose gel possess long-term (> 3 weeks) viability, significant self-assembly to form tissue like aggregates, low lactate dehydrogenase release and high albumin synthesis. These were in contrast to 2D culture of hepatocytes. Our results suggest that the 3D culture of hepatocytes in agarose gel favors aggregate formation of functionally active cells and would be useful for liver transplantation as well as to analyze hepatocytes biology.

Original languageEnglish (US)
Pages (from-to)209-213
Number of pages5
JournalBiotechnology Letters
Volume21
Issue number3
DOIs
StatePublished - Jan 1 1999

Keywords

  • 3D culture
  • Agarose gel
  • Aggregates
  • Hepatocytes

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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