Analysis of D2d

a D-region class I gene

Mary Lynne Hedley, Stephen W. Hunt, Kurt A. Brorson, Jennifer S. Andris, Leroy Hood, James Forman, Philip W. Tucker

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The mouse major histocompatibility complex is composed of several genes arranged into the K, D, Qa, and Tla regions. The D region of the BALB/c mouse includes genes D2d, D3d, and D4d, in addition to H-2Ddand H-2Ld. We have determined the DNA sequence of the D2dgene and compared it with the known sequences of several class I genes. The exon/intron structure of the D2dgene is similar to other class I genes. It also contains similar 5′ regulatory elements. A frameshift occurs in exon seven, resulting in a gene product with a truncated cytoplasmic tail. To examine the surface expression of the D2d molecule, we generated an exon-shuffled construct containing the promoter and exons 1-3, encoding the signal peptide, α1, and α2 external domains of the D2dgene linked to exons 4-8, encoding the α3, transmembrane and cytoplasmic domains, of the H-2Ddgene. The construct was transfected into mouse L cells, and a protein was detected at the cell surface by a monoclonal antibody (mAb) specific for the α3 domain of H-2Dd, as well as by other class I-specific mAbs. Although D2d is expressed at low levels, it may be a functional class I gene that most probably evolved from a Qa region gene.

Original languageEnglish (US)
Pages (from-to)359-365
Number of pages7
JournalImmunogenetics
Volume29
Issue number6
DOIs
StatePublished - Jun 1 1989

Fingerprint

MHC Class I Genes
Exons
Genes
Protein Sorting Signals
Major Histocompatibility Complex
Introns
Tail
Monoclonal Antibodies
Proteins

ASJC Scopus subject areas

  • Immunology
  • Genetics

Cite this

Hedley, M. L., Hunt, S. W., Brorson, K. A., Andris, J. S., Hood, L., Forman, J., & Tucker, P. W. (1989). Analysis of D2d: a D-region class I gene. Immunogenetics, 29(6), 359-365. https://doi.org/10.1007/BF00375863

Analysis of D2d : a D-region class I gene. / Hedley, Mary Lynne; Hunt, Stephen W.; Brorson, Kurt A.; Andris, Jennifer S.; Hood, Leroy; Forman, James; Tucker, Philip W.

In: Immunogenetics, Vol. 29, No. 6, 01.06.1989, p. 359-365.

Research output: Contribution to journalArticle

Hedley, ML, Hunt, SW, Brorson, KA, Andris, JS, Hood, L, Forman, J & Tucker, PW 1989, 'Analysis of D2d: a D-region class I gene', Immunogenetics, vol. 29, no. 6, pp. 359-365. https://doi.org/10.1007/BF00375863
Hedley ML, Hunt SW, Brorson KA, Andris JS, Hood L, Forman J et al. Analysis of D2d: a D-region class I gene. Immunogenetics. 1989 Jun 1;29(6):359-365. https://doi.org/10.1007/BF00375863
Hedley, Mary Lynne ; Hunt, Stephen W. ; Brorson, Kurt A. ; Andris, Jennifer S. ; Hood, Leroy ; Forman, James ; Tucker, Philip W. / Analysis of D2d : a D-region class I gene. In: Immunogenetics. 1989 ; Vol. 29, No. 6. pp. 359-365.
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