Analysis of NPR-1 reveals a circuit mechanism for behavioral quiescence in C.elegans

Seungwon Choi; Marios Chatzigeorgiou; Kelsey P. Taylor; William R. Schafer; Joshua M. Kaplan

doi:10.1016/j.neuron.2013.04.002

Analysis of NPR-1 reveals a circuit mechanism for behavioral quiescence in C.elegans

Seungwon Choi, Marios Chatzigeorgiou, Kelsey P. Taylor, William R. Schafer, Joshua M. Kaplan

Research output: Contribution to journal › Article › peer-review

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Abstract

Animals undergo periods of behavioral quiescence and arousal in response to environmental, circadian,or developmental cues. During larval molts, C.elegans undergoes a period of profound behavioral quiescence termed lethargus. Locomotion quiescence during lethargus was abolished in mutants lacking a neuropeptide receptor (NPR-1) and was reduced in mutants lacking NPR-1 ligands (FLP-18 and FLP-21). Wild-type strains are polymorphic for the npr-1 gene, and their lethargus behavior varies correspondingly. Locomotion quiescence and arousal were mediated by decreased and increased secretion of an arousal neuropeptide (PDF-1) from central neurons. PDF receptors (PDFR-1) expressed in peripheral mechanosensory neurons enhanced touch-evoked calcium transients. Thus, a central circuit stimulates arousal from lethargus by enhancing the sensitivity of peripheral mechanosensory neurons in the body. These results define a circuit mechanism controlling a developmentally programmed form of quiescence

Original language	English (US)
Pages (from-to)	869-880
Number of pages	12
Journal	Neuron
Volume	78
Issue number	5
DOIs	https://doi.org/10.1016/j.neuron.2013.04.002
State	Published - Jun 5 2013
Externally published	Yes

ASJC Scopus subject areas

General Neuroscience

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10.1016/j.neuron.2013.04.002

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title = "Analysis of NPR-1 reveals a circuit mechanism for behavioral quiescence in C.elegans",

abstract = "Animals undergo periods of behavioral quiescence and arousal in response to environmental, circadian,or developmental cues. During larval molts, C.elegans undergoes a period of profound behavioral quiescence termed lethargus. Locomotion quiescence during lethargus was abolished in mutants lacking a neuropeptide receptor (NPR-1) and was reduced in mutants lacking NPR-1 ligands (FLP-18 and FLP-21). Wild-type strains are polymorphic for the npr-1 gene, and their lethargus behavior varies correspondingly. Locomotion quiescence and arousal were mediated by decreased and increased secretion of an arousal neuropeptide (PDF-1) from central neurons. PDF receptors (PDFR-1) expressed in peripheral mechanosensory neurons enhanced touch-evoked calcium transients. Thus, a central circuit stimulates arousal from lethargus by enhancing the sensitivity of peripheral mechanosensory neurons in the body. These results define a circuit mechanism controlling a developmentally programmed form of quiescence",

author = "Seungwon Choi and Marios Chatzigeorgiou and Taylor, {Kelsey P.} and Schafer, {William R.} and Kaplan, {Joshua M.}",

note = "Funding Information: We thank the following for strains, advice, reagents, and comments on the manuscript: Cori Bargmann for NPR-1 sensory rescue and rat TRPV1 transgenes, Mario de Bono for flp-18 mutants, the Caenorhabditis Genetics Center (CGC) and S. Mitani for strains, and members of the Kaplan lab for comments on the manuscript. This work was supported by a Kwanjeong Educational Foundation Predoctoral Fellowship (S.C.), and by research grants to J.K. (NIH DK80215), and to W.S. (MRC MC-A022-5PB9). ",

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AU - Schafer, William R.

AU - Kaplan, Joshua M.

N1 - Funding Information: We thank the following for strains, advice, reagents, and comments on the manuscript: Cori Bargmann for NPR-1 sensory rescue and rat TRPV1 transgenes, Mario de Bono for flp-18 mutants, the Caenorhabditis Genetics Center (CGC) and S. Mitani for strains, and members of the Kaplan lab for comments on the manuscript. This work was supported by a Kwanjeong Educational Foundation Predoctoral Fellowship (S.C.), and by research grants to J.K. (NIH DK80215), and to W.S. (MRC MC-A022-5PB9).

PY - 2013/6/5

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N2 - Animals undergo periods of behavioral quiescence and arousal in response to environmental, circadian,or developmental cues. During larval molts, C.elegans undergoes a period of profound behavioral quiescence termed lethargus. Locomotion quiescence during lethargus was abolished in mutants lacking a neuropeptide receptor (NPR-1) and was reduced in mutants lacking NPR-1 ligands (FLP-18 and FLP-21). Wild-type strains are polymorphic for the npr-1 gene, and their lethargus behavior varies correspondingly. Locomotion quiescence and arousal were mediated by decreased and increased secretion of an arousal neuropeptide (PDF-1) from central neurons. PDF receptors (PDFR-1) expressed in peripheral mechanosensory neurons enhanced touch-evoked calcium transients. Thus, a central circuit stimulates arousal from lethargus by enhancing the sensitivity of peripheral mechanosensory neurons in the body. These results define a circuit mechanism controlling a developmentally programmed form of quiescence

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Analysis of NPR-1 reveals a circuit mechanism for behavioral quiescence in C.elegans

Abstract

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